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Aconitase 2 antibody, Internal

Anti-Aconitase 2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88196
Anti-Aconitase 2 antibody, Internal used in Western Blot (WB). GTX88196

Cat. No. GTX88196

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Aconitase 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.01-0.03μg/ml
ICC/IF Assay dependent
IHC-P 3-5μg/ml

Note :

IHC-P
Human Breast shows mitochondrial staining in the epithelial cells of the ducts.
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88196-PEP).

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS pH7.3, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QHVDVSPTSQRLQ, from the internal region of the protein sequence according to NP_001089.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aconitase 2 , ACONM , HEL-S-284 , ICRD , OCA8 , OPA9

Cellular Localization

Mitochondrion

Background

The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Aconitase 2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88196

GTX88196 IHC-P Image

IHC-P analysis of human breast using GTX88196 Aconitase 2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml

Anti-Aconitase 2 antibody, Internal used in Western Blot (WB). GTX88196

GTX88196 WB Image

WB analysis of heart lysate using GTX88196 Aconitase 2 antibody, Internal.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399