GeneTex
United States (US)

Aconitase 2 antibody, Internal

GTX88196_2490_WB_18121411_531.jpg
GTX88196_1045_IHC-P_18121411_865.jpg

Cat No. GTX88196

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.01-0.03μg/ml
ICC/IF Assay dependent
IHC-P 3-5μg/ml

Note :

Human Breast shows mitochondrial staining in the epithelial cells of the ducts.
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88196-PEP).

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-QHVDVSPTSQRLQ, from the internal region of the protein sequence according to NP_001089.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ACO2 Antibody , ICRD Antibody , ACONM Antibody

Background

The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq, Jul 2008]
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Database

Research Area

DATA IMAGES
GTX88196_2490_WB_18121411_531.jpg

GTX88196 WB Image

WB analysis of heart lysate using GTX88196 Aconitase 2 antibody, Internal.
Dilution : 0.01µg/ml
Loading : 35µg protein in RIPA buffer

GTX88196_1045_IHC-P_18121411_865.jpg

GTX88196 IHC-P Image

IHC-P analysis of human breast using GTX88196 Aconitase 2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml