Aconitase 2 antibody [N1N3]

Name: Aconitase 2 antibody [N1N3]
Brand: GeneTex
SKU: GTX114233
Price: 399.00 USD

Anti-Aconitase 2 antibody [N1N3] used in Western Blot (WB). GTX114233

Anti-Aconitase 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114233

Anti-Aconitase 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX114233

Anti-Aconitase 2 antibody [N1N3] used in Immunoprecipitation (IP). GTX114233

Cat. No. GTX114233

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat, Zebrafish, Chicken
Package	100 μl ($399), 25 μl ($169)

See all Aconitase 2 products

View product citations for antibody GTX114233 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

Mouse brain , Jurkat , 293T , A431 , HeLa , HepG2 , rat brain

Predict Reactivity

Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Aconitase 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619443

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aconitase 2 , ACONM , HEL-S-284 , ICRD , OCA8 , OPA9

Cellular Localization

Mitochondrion

Background

The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq]

Database

Gene ID: 50 ACO2
UniProt: Q99798 ACO2

Research Area

DATA IMAGES

GTX114233 WB Image

Aconitase 2 antibody detects Aconitase 2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody (GTX114233) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114233 ICC/IF Image

Aconitase 2 antibody [N1N3] detects Aconitase 2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: Aconitase 2 protein stained by Aconitase 2 antibody [N1N3] (GTX114233) diluted at 1:1000.
Red: MitoTrackerR Red CMXRos, a mitochondria tracker.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX114233 IHC-P Image

Aconitase 2 antibody [N1N3] detects Aconitase 2 protein at mitochondria on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Aconitase 2 antibody [N1N3] (GTX114233) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114233 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [N1N3] (GTX114233) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114233 IP Image

Immunoprecipitation of Aconitase 2 protein from HeLa whole cell extracts using 5 μg of Aconitase 2 antibody [N1N3] (GTX114233).
Western blot analysis was performed using Aconitase 2 antibody [N1N3] (GTX114233).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX114233 IHC-P Image

Aconitase 2 antibody [N1N3] detects Aconitase 2 protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Aconitase 2 antibody [N1N3] (GTX114233) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114233 IHC-P Image

Aconitase 2 antibody [N1N3] detects Aconitase 2 protein at mitochondria on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
Aconitase 2 antibody [N1N3] (GTX114233) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114233 WB Image

Sample (30 μg of whole cell lysate)
A: JurKat
7.5% SDS PAGE
Aconitase 2 antibody
GTX114233 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114233 IHC-P Image

GTX114233 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
7.5% SDS PAGE
GTX114233 diluted at 1:1000

GTX114233 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Aconitase 2 antibody [N1N3] (GTX114233) at 1:300 dilution.

GTX114233 WB Image

Sample (20 μg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX114233 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114233 WB Image

The data was published in the journal Biomolecules in 2019. PMID: 31614639

REFERENCE

Application Reference

Garc?a-Arroyo FE et al. Biomolecules 2019; 9 (10) Allopurinol Prevents the Lipogenic Response Induced by an Acute Oral Fructose Challenge in Short-Term Fructose Fed Rats.

Mizushima S et al. Development 2014; 141 (19) : 3799-806 The birth of quail chicks after intracytoplasmic sperm injection.

Application : WB

Reactivity : Chicken

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REVIEW

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Application Reference

Garc?a-Arroyo FE et al. Biomolecules 2019; 9 (10) Allopurinol Prevents the Lipogenic Response Induced by an Acute Oral Fructose Challenge in Short-Term Fructose Fed Rats.

Mizushima S et al. Development 2014; 141 (19):3799-806 The birth of quail chicks after intracytoplasmic sperm injection.

Application : WB

Reactivity : Chicken

SDS
Glycerol.pdf
Thimerosal.pdf

Package	List Price ($)
100 μl	$ 399
25 μl	$ 169
Qty

Aconitase 2 antibody [N1N3]

Cat. No. GTX114233

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Positive Control

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX114233 WB Image

GTX114233 ICC/IF Image

GTX114233 IHC-P Image

GTX114233 WB Image

GTX114233 IP Image

GTX114233 IHC-P Image

GTX114233 IHC-P Image

GTX114233 WB Image

GTX114233 IHC-P Image

GTX114233 WB Image

GTX114233 IHC-P Image

GTX114233 WB Image

GTX114233 WB Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.