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Aconitase 2 antibody

Anti-Aconitase 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX02248
Anti-Aconitase 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX02248
Anti-Aconitase 2 antibody used in Western Blot (WB). GTX02248
Anti-Aconitase 2 antibody used in Immunoprecipitation (IP). GTX02248

Cat No. GTX02248

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
IHC-P 1:500-1:2000
IP 1-4 μl/mg of lysate
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.2 μm filtered PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Human ACO2 / Aconitase 2 protein

Purification

Purified by Protein A and Antigen Affinity

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

aconitase 2 , ACONM , HEL-S-284 , ICRD , OCA8 , OPA9

Cellular Localization

Mitochondrion

Background

The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Aconitase 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX02248

GTX02248 IHC-P Image

IHC-P analysis of human kidney tissue section using GTX02248 Aconitase 2 antibody.
Dilution : 1:1000

Anti-Aconitase 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX02248

GTX02248 IHC-P Image

IHC-P analysis of human skeletal muscle tissue section using GTX02248 Aconitase 2 antibody.
Dilution : 1:1000

Anti-Aconitase 2 antibody used in Western Blot (WB). GTX02248

GTX02248 WB Image

WB analysis of various samples using GTX02248 Aconitase 2 antibody.
Lane A : Rat heart tissue lysate
Lane B : Rat brain tissue lysate
Lane C : Mouse brain tissue lysate
Lane D : Jurkat whole cell lysate
Dilution : 1:500
Loading : 30 μg

Anti-Aconitase 2 antibody used in Immunoprecipitation (IP). GTX02248

GTX02248 IP Image

IP analysis of K562 whole cell lysate using GTX02248 Aconitase 2 antibody.
IP antibody : 4 μl per 0.5 mg total whole cell lysate
Dilution : 1:100
50 % Protein G agarose : 15 μl

REFERENCE

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REVIEW

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Package List Price ($)
$ 339