GeneTex
United States (US)

Acta1b antibody

GTX124484_39694_IHC_Z_2_18121410_674.jpg
GTX124484_39694_IHC_18121410_681.jpg
GTX124484_42060_20150909_IHC-Wm_Z_18121410_677.jpg
GTX124484_42060_20150702_WB_Z_heart_19021719_523.jpg

Cat No. GTX124484

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Wm, IHC
Reactivity Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm 1:50-1:500
IHC 1:100-1:1000
Not tested in other applications.

Positive Control

whole zebrafish
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Actin, Alpha 1B, Skeletal Muscle,Actc,Actc1,Cfk,Hm:Zeh0631,Zeh0631,Acta1B

Background

Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

Research Area

DATA IMAGES
GTX124484_39694_IHC_Z_2_18121410_674.jpg

GTX124484 IHC Image

Immunohistochemical analysis of agarose-embedded zebrafish embryo tail section, using acta1b (GTX124484) antibody at 1:200 dilution.

GTX124484_39694_IHC_18121410_681.jpg

GTX124484 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using acta1b (GTX124484) antibody at 1:300 dilution.

GTX124484_42060_20150909_IHC-Wm_Z_18121410_677.jpg

GTX124484 IHC-Wm Image

Acta1b antibody detects Acta1b protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Acta1b antibody (GTX124484) dilution: 1:50.

GTX124484_42060_20150702_WB_Z_heart_19021719_523.jpg

GTX124484 WB Image

Acta1b antibody detects Acta1b protein by Western blot analysis. Zebrafish tissue extracts (30 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with Acta1b antibody (GTX101362) at a dilution of 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 389
$ 199