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Acta1b antibody

Anti-Acta1b antibody used in IHC (Whole mount) (IHC-Wm). GTX124484
Anti-Acta1b antibody used in IHC (Paraffin sections) (IHC-P). GTX124484
Anti-Acta1b antibody used in Immunohistochemistry (IHC). GTX124484
Anti-Acta1b antibody used in Western Blot (WB). GTX124484

Cat. No. GTX124484

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm, IHC

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Acta1b products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm 1:50-1:500
IHC 1:100-1:1000
Not tested in other applications.

Positive Control

zebrafish heart

Predict Reactivity

Human, Mouse, Rat, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Acta1b

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167993

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin alpha 1, skeletal muscle b , actc , actc1 , cfk , hm:zeh0631 , zeh0631

Database

Research Area

DATA IMAGES

Anti-Acta1b antibody used in IHC (Whole mount) (IHC-Wm). GTX124484

GTX124484 IHC-Wm Image

Acta1b antibody detects Acta1b protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Acta1b antibody (GTX124484) dilution: 1:50.
Anti-Acta1b antibody used in IHC (Paraffin sections) (IHC-P). GTX124484

GTX124484 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using acta1b (GTX124484) antibody at 1:300 dilution.

Anti-Acta1b antibody used in Immunohistochemistry (IHC). GTX124484

GTX124484 IHC Image

Immunohistochemical analysis of agarose-embedded zebrafish embryo tail section, using acta1b (GTX124484) antibody at 1:200 dilution.

Anti-Acta1b antibody used in Western Blot (WB). GTX124484

GTX124484 WB Image

Acta1b antibody detects Acta1b protein by Western blot analysis. Zebrafish tissue extracts (30 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with Acta1b antibody (GTX101362) at a dilution of 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169