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Acta2 antibody

Anti-Acta2 antibody used in Western Blot (WB). GTX124505
Anti-Acta2 antibody used in Western Blot (WB). GTX124505
Anti-Acta2 antibody used in IHC (Paraffin sections) (IHC-P). GTX124505
Anti-Acta2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124505

Cat. No. GTX124505

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, IHC-Wm

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all alpha Smooth Muscle Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
Not tested in other applications.

Positive Control

zebrafish muscle , whole zebrafish

Predict Reactivity

Human (100%)| Mouse (100%)| Rat (100%)| Rabbit (100%)| Bovine (100%)| Chicken (100%)| Pig (100%)| Rhesus Monkey (100%)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Acta2

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166745

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin alpha 2, smooth muscle , wu:fb63d03

Database

Research Area

DATA IMAGES

Anti-Acta2 antibody used in Western Blot (WB). GTX124505

GTX124505 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Acta2 antibody (GTX124505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Acta2 antibody used in Western Blot (WB). GTX124505

GTX124505 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with  alpha Smooth Muscle Actin antibody (GTX124505) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Acta2 antibody used in IHC (Paraffin sections) (IHC-P). GTX124505

GTX124505 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Acta2 (GTX124505) antibody at 1:300 dilution.

Anti-Acta2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124505

GTX124505 IHC-Wm Image

Acta2 antibody detects Acta2 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Acta2 antibody (GTX124505) dilution: 1:100.

REFERENCE

Application Reference

Shin K et al. bioRxiv 2024; Harnessing the regenerative potential of interleukin11 to enhance heart repair.
WA Sebastian et al. J Hum Genet 2024; Cardiac manifestations of human ACTA2 variants recapitulated in a zebrafish model
Application : IHC-Fr
Zada A et al. Hum Mol Genet 2023; 32 (1) : 151-160 The long Filamin-A isoform is required for intestinal development and motility: implications for chronic intestinal pseudo-obstruction.
Application : IHC-P
Reactivity : Zebrafish
Allanki S et al. Sci Adv 2021; 7 (37) : eabg6497 Interleukin-11 signaling promotes cellular reprogramming and limits fibrotic scarring during tissue regeneration.
Application : IHC-Fr
Reactivity : Zebrafish
Chen HH et al. iScience 2021; 24 (9) : 103086 DDX3 modulates the tumor microenvironment via its role in endoplasmic reticulum-associated translation.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169