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Acta2 antibody

Anti-Acta2 antibody used in IHC (Paraffin sections) (IHC-P). GTX124505
Anti-Acta2 antibody used in Western Blot (WB). GTX124505
Anti-Acta2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124505

Cat No. GTX124505

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC-Wm

Reactivity

Zebrafish
Package
200 μl ($409),
50 μl ($219)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
IHC-Wm 1:100-1:500
Not tested in other applications.

Positive Control

Adult zebrafish , Zebrafish eye

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Acta2

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

actin alpha 2, smooth muscle , wu:fb63d03

Database

Research Area

DATA IMAGES

Anti-Acta2 antibody used in IHC (Paraffin sections) (IHC-P). GTX124505

GTX124505 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Acta2 (GTX124505) antibody at 1:300 dilution.

Anti-Acta2 antibody used in Western Blot (WB). GTX124505

GTX124505 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with  alpha Smooth Muscle Actin antibody (GTX124505) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Acta2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124505

GTX124505 IHC-Wm Image

Acta2 antibody detects Acta2 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Acta2 antibody (GTX124505) dilution: 1:100.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 409
$ 219