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Actin Gamma 1 antibody [2A3]

KO/KD
Anti-Actin Gamma 1 antibody [2A3] used in Western Blot (WB). GTX03493
Anti-Actin Gamma 1 antibody [2A3] used in Western Blot (WB). GTX03493
Anti-Actin Gamma 1 antibody [2A3] used in Western Blot (WB). GTX03493
Anti-Actin Gamma 1 antibody [2A3] used in Western Blot (WB). GTX03493
Anti-Actin Gamma 1 antibody [2A3] used in Western Blot (WB). GTX03493

Cat. No. GTX03493

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2A3

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μg ($399)
See all Actin Gamma 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100 - 1:1000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Product Note

This antibody is highly specific for actin gamma and does not react with other actin isoforms (Dugina et al. 2009).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to the N-terminal nonapeptide of gamma-cytoplasmic actin, coupled to KLH.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin gamma 1 , ACT , ACTG , DFNA20 , DFNA26 , HEL-176

Cellular Localization

Cytoplasm, cytoskeleton

Background

Actins are highly conserved proteins that are involved in various types of cell motility and in maintenance of the cytoskeleton. Three main groups of actin isoforms have been identified in vertebrate animals: alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. Actin gamma 1, encoded by this gene, is a cytoplasmic actin found in all cell types. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss and also with Baraitser-Winter syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017]

Database

  • Gene ID: 71 ACTG1
  • UniProt: P63261 ACTG1

Research Area

DATA IMAGES

Anti-Actin Gamma 1 antibody [2A3] used in Western Blot (WB). GTX03493

GTX03493 WB Image

WB analysis of COS-7 cell lysate using GTX03493 Actin Gamma 1 antibody [2A3].

Anti-Actin Gamma 1 antibody [2A3] used in Western Blot (WB). GTX03493

GTX03493 WB Image

WB analysis of NIH3T3 cell lysate using GTX03493 Actin Gamma 1 antibody [2A3].

Anti-Actin Gamma 1 antibody [2A3] used in Western Blot (WB). GTX03493

GTX03493 WB Image

WB analysis of various sample lysates using GTX03493 Actin Gamma 1 antibody [2A3].

Anti-Actin Gamma 1 antibody [2A3] used in Western Blot (WB). GTX03493

GTX03493 WB Image

WB analysis of HeLa cell lysate using GTX03493 Actin Gamma 1 antibody [2A3].

Anti-Actin Gamma 1 antibody [2A3] used in Western Blot (WB). GTX03493

GTX03493 WB Image

KO/KD

WB analysis of knockout HeLa and wild type HeLa (WT) whole cell lysates using GTX03493 Actin Gamma 1 antibody [2A3]. Panel B is an internal control.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399