GeneTex
United States (US)

Actin Gamma 1 antibody

GTX101794_WB_19011506_869.jpg
GTX101794_40128_WB_M_19011506_191.jpg
GTX101794_40128_IHC_19011506_443.jpg
GTX101794_40128_20151231_WB_R_19011506_107.jpg

Cat No. GTX101794

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

A431 , NIH-3T3 , JC , BCL-1 , PC-12 , Rat2

Predict Reactivity

Bovine, Dog, Chicken, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Actin Gamma 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ACT antibody, ACTG antibody, DFNA20 antibody, DFNA26 antibody, ACTG1 antibody, cytoskeletal gamma-actin antibody, "actin, cytoplasmic 2 antibody", "actin, gamma 1 antibody"

Cellular Localization

Cytoplasm , cytoskeleton

Background

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101794_WB_19011506_869.jpg

GTX101794 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX101794 diluted at 1:1000

GTX101794_40128_WB_M_19011506_191.jpg

GTX101794 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
10% SDS PAGE
GTX101794 diluted at 1:1000

GTX101794_40128_IHC_19011506_443.jpg

GTX101794 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human Spleen, using gamma Actin(GTX101794) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101794_40128_20151231_WB_R_19011506_107.jpg

GTX101794 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Sarcomeric Actin antibody (GTX101794) diluted at 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169