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Actin Gamma 1 antibody

Anti-Actin Gamma 1 antibody used in Western Blot (WB). GTX101794
Anti-Actin Gamma 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101794
Anti-Actin Gamma 1 antibody used in Western Blot (WB). GTX101794
Anti-Actin Gamma 1 antibody used in Western Blot (WB). GTX101794
Anti-Actin Gamma 1 antibody used in Western Blot (WB). GTX101794
Anti-Actin Gamma 1 antibody used in Western Blot (WB). GTX101794
Anti-Actin Gamma 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101794
Anti-Actin Gamma 1 antibody used in Western Blot (WB). GTX101794
Anti-Actin Gamma 1 antibody used in Western Blot (WB). GTX101794
Anti-Actin Gamma 1 antibody used in Western Blot (WB). GTX101794
Anti-Actin Gamma 1 antibody used in Western Blot (WB). GTX101794

Cat. No. GTX101794

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all Actin Gamma 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

NIH-3T3 , JC , BCL-1 , A431 , PC-12 , Rat2

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Actin Gamma 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036153

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin gamma 1 , ACT , ACTG , DFNA20 , DFNA26 , HEL-176

Cellular Localization

Cytoplasm , cytoskeleton

Background

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells. [provided by RefSeq]

Database

  • Gene ID: 71 ACTG1
  • UniProt: P63261 ACTG1

Research Area

DATA IMAGES

Anti-Actin Gamma 1 antibody used in Western Blot (WB). GTX101794

GTX101794 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Sarcomeric Actin antibody (GTX101794) diluted at 1:1000.

Anti-Actin Gamma 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101794

GTX101794 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human Spleen, using gamma Actin(GTX101794) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Actin Gamma 1 antibody used in Western Blot (WB). GTX101794

GTX101794 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX101794 diluted at 1:1000

Anti-Actin Gamma 1 antibody used in Western Blot (WB). GTX101794

GTX101794 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
10% SDS PAGE
GTX101794 diluted at 1:1000

Anti-Actin Gamma 1 antibody used in Western Blot (WB). GTX101794

GTX101794 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Actin Gamma 1 antibody (GTX101794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Actin Gamma 1 antibody used in Western Blot (WB). GTX101794

GTX101794 WB Image

PG-4 whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Actin Gamma 1 antibody (GTX101794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Actin Gamma 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101794

GTX101794 IHC-P Image

Actin Gamma 1 antibody detects Actin Gamma 1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded cat intestine.
Actin Gamma 1 stained by Actin Gamma 1 antibody (GTX101794) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Actin Gamma 1 antibody used in Western Blot (WB). GTX101794

GTX101794 WB Image

The data was published in the journal J Diabetes Res in 2018.PMID: 29955616

Anti-Actin Gamma 1 antibody used in Western Blot (WB). GTX101794

GTX101794 WB Image

The data was published in the journal Cancers (Basel) in 2019.PMID: 31430901

Anti-Actin Gamma 1 antibody used in Western Blot (WB). GTX101794

GTX101794 WB Image

The data was published in the journal Cancers (Basel) in 2019.PMID: 31430901

Anti-Actin Gamma 1 antibody used in Western Blot (WB). GTX101794

GTX101794 WB Image

The data was published in the journal Cancers (Basel) in 2019.PMID: 31430901

REFERENCE

Application Reference

Chen YH et al. Cancers (Basel) 2019; 11 (8) Maintenance BEZ235 Treatment Prolongs the Therapeutic Effect of the Combination of BEZ235 and Radiotherapy for Colorectal Cancer.
Application : WB
Reactivity : Human
Chang YC et al. Cancer Res 2019; Therapeutic Targeting of Aldolase A Interactions Inhibits Lung Cancer Metastasis and Prolongs Survival.
Xie Y et al. Anal Biochem 2019; 577 : 1月13日 Quantitative in situ proximity ligation assays examining protein interactions and phosphorylation during smooth muscle contractions.
Application : IHC-Fr
Reactivity : Mouse
Li W et al. J Diabetes Res 2018; 2018 : 8743874 Contractile Protein Expression and Phosphorylation and Contractility of Gastric Smooth Muscles from Obese Patients and Patients with Obesity and Diabetes.
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169