GeneTex

  • Country / Location Selection

United States (US)

Actin antibody [AC-40]

Anti-Actin antibody [AC-40] used in IHC (Frozen sections) (IHC-Fr). GTX11003
Anti-Actin antibody [AC-40] used in Western Blot (WB). GTX11003
Anti-Actin antibody [AC-40] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11003

Cat. No. GTX11003

Reference 23
6

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AC-40

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-Fr, ELISA

Reactivity

 Human, Mouse, Rat, Rabbit, Goat, Sheep, Bovine, Dog, Hamster, Chicken, Guinea pig, Pig, Monkey, Carp, Snail, Viper, Xenopus
Package
100 μl ($419)
See all Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:200
IHC-Fr Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

human or chicken fibroblast

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

synthetic actin C-terminal peptide Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe, attached to a Multiple Antigen Peptide (MAP) backbone.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_384201

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

actin alpha 1, skeletal muscle , ACTA , ASMA , CFTD , CFTD1 , CFTDM , MPFD , NEM1 , NEM2 , NEM3 , SHPM

Cellular Localization

Cytoplasm, cytoskeleton

Background

The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.

Database

  • Gene ID: 58 ACTA1
  • UniProt: P68133 ACTA1

Research Area

DATA IMAGES

Anti-Actin antibody [AC-40] used in IHC (Frozen sections) (IHC-Fr). GTX11003

GTX11003 IHC-Fr Image

IHC-Fr analysis of human tongue tissue using GTX11003 Actin antibody [AC-40].

Anti-Actin antibody [AC-40] used in Western Blot (WB). GTX11003

GTX11003 WB Image

WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK, and (9) MDCK lysates using Actin antibody [AC-40] at 1:500.

Anti-Actin antibody [AC-40] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11003

GTX11003 ICC/IF Image

ICC/IF analysis of Rat2 cells using GTX11003 Actin antibody [AC-40] at 1:250 (red) with DAPI (blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.

REFERENCE

Application Reference

Guo CJ et al. Mol Ther Nucleic Acids 2023; 31 : 763-778 Exosome-mediated inhibition of microRNA-449a promotes the amplification of mouse retinal progenitor cells and enhances their transplantation in retinal degeneration mouse models.
Zhang Y et al. Cell Death Discov 2023; 9 (1) : 82 Downregulation of KLF10 contributes to the regeneration of survived renal tubular cells in cisplatin-induced acute kidney injury via ZBTB7A-KLF10-PTEN axis.
Wei MF et al. Oxid Med Cell Longev 2022; 2022 : 7957255 Atorvastatin Attenuates Radiotherapy-Induced Intestinal Damage through Activation of Autophagy and Antioxidant Effects.
Application : WB
Reactivity : Mouse
Yu-Xuan Ma et al. Bioact Mater 2022; 9 : 475-490 Silicified collagen scaffold induces semaphorin 3A secretion by sensory nerves to improve in-situ bone regeneration.
Application : WB
Reactivity : Rat
Alexandra G Moyzis et al. Cells 2022; 11 (9) Mcl-1 Differentially Regulates Autophagy in Response to Changes in Energy Status and Mitochondrial Damage.
Application : WB
Show More +
Submit a Reference

REVIEW

Actin antibody [AC-40] Cat. No. GTX11003

Rating
( Average 5 based on 6 users reviews)
Application
Western Blot(WB)
( Average 5 based on 6 users reviews)
Anti-Actin antibody [AC-40] used in Western Blot (WB). GTX11003
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29955155
Anti-Actin antibody [AC-40] used in Western Blot (WB). GTX11003
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Evid Based Complement Alternat Med in 2012.PMID: 23320038
Anti-Actin antibody [AC-40] used in Western Blot (WB). GTX11003
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Evid Based Complement Alternat Med in 2012.PMID: 23320038
Anti-Actin antibody [AC-40] used in Western Blot (WB). GTX11003
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29955155
Anti-Actin antibody [AC-40] used in Western Blot (WB). GTX11003
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29955155
Anti-Actin antibody [AC-40] used in Western Blot (WB). GTX11003
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014. PMID: 25089569
Show More +
Submit a Review
Application Reference
Guo CJ et al. Mol Ther Nucleic Acids 2023; 31:763-778 Exosome-mediated inhibition of microRNA-449a promotes the amplification of mouse retinal progenitor cells and enhances their transplantation in retinal degeneration mouse models.
Zhang Y et al. Cell Death Discov 2023; 9 (1):82 Downregulation of KLF10 contributes to the regeneration of survived renal tubular cells in cisplatin-induced acute kidney injury via ZBTB7A-KLF10-PTEN axis.
Wei MF et al. Oxid Med Cell Longev 2022; 2022:7957255 Atorvastatin Attenuates Radiotherapy-Induced Intestinal Damage through Activation of Autophagy and Antioxidant Effects.
Application : WB
Reactivity : Mouse
Yu-Xuan Ma et al. Bioact Mater 2022; 9:475-490 Silicified collagen scaffold induces semaphorin 3A secretion by sensory nerves to improve in-situ bone regeneration.
Application : WB
Reactivity : Rat
Alexandra G Moyzis et al. Cells 2022; 11 (9) Mcl-1 Differentially Regulates Autophagy in Response to Changes in Energy Status and Mitochondrial Damage.
Application : WB
Heng Liu et al. Exp Ther Med 2021; 22 (3):945 MicroRNA-301a-3p promotes triple-negative breast cancer progression through downregulating MEOX2.
Application : WB
Reactivity : Human
Bease AG et al. J Mol Biol 2021; 433 (19):167200 Activity of Lymphostatin, A Lymphocyte Inhibitory Virulence Factor of Pathogenic Escherichia coli, is Dependent on a Cysteine Protease Motif.
Application : WB
Reactivity : Mouse
Wang HC et al. Int J Mol Sci 2021; 22 (5) Silencing DNA Polymerase β Induces Aneuploidy as a Biomarker of Poor Prognosis in Oral Squamous Cell Cancer.
Application : WB
Reactivity : Human
Moyzis AG et al. J Mol Cell Cardiol 2020; 146:109-120 Mcl-1-mediated mitochondrial fission protects against stress but impairs cardiac adaptation to exercise.
Application : WB
Gronseth E et al. PLoS One 2020; 15 (7):e0235852 Astrocytes influence medulloblastoma phenotypes and CD133 surface expression.
Application : WB
Reactivity : Rat
Zhang Y et al. Free Radic Biol Med 2020; 158:60-73 Arginine methylation of APE1 promotes its mitochondrial translocation to protect cells from oxidative damage.
Application : WB
Reactivity : Human
Wang MJ et al. Med Sci Monit 2019; 25:4627-4638 Levetiracetam Protects Against Cognitive Impairment of Subthreshold Convulsant Discharge Model Rats by Activating Protein Kinase C (PKC)-Growth-Associated Protein 43 (GAP-43)-Calmodulin-Dependent Protein Kinase (CaMK) Signal Transduction Pathway.
Application : WB
Reactivity : Rat
Kuo CC et al. Theranostics 2019; 9 (9):2526-2540 Metastatic Colorectal Cancer Rewrites Metabolic Program Through a Glut3-YAP-dependent Signaling Circuit.
Application : WB
Reactivity : Human
Liu Y et al. Int Immunopharmacol 2018; 62:270-276 Activation of PPARγ by Curcumin protects mice from ischemia/reperfusion injury induced by orthotopic liver transplantation via modulating polarization of Kupffer cells.
Application : WB
Reactivity : Mouse
Chang CC et al. Sci Rep 2018; 8 (1):9786 The role of sentrin-specific protease 2 substrate recognition in TGF-β-induced tumorigenesis.
Application : WB
Reactivity : Human
Zhang GN et al. Cancer Lett 2018; (Epub) Epidermal growth factor receptor (EGFR) inhibitor PD153035 reverses ABCG2-mediated multidrug resistance in non-small cell lung cancer: In vitro and in vivo.
Reactivity : Human
Wei SD et al. Mol Med Rep 2018; (Epub) Investigation of the expressions of MMPs and TIMPs between isogeneic and allogeneic rat aortic transplantation.
Application : WB
Chien T et al. Mol Psychiatry 2018; (Epub) GSK3β negatively regulates TRAX, a scaffold protein implicated in mental disorders, for NHEJ-mediated DNA repair in neurons.
Application : WB
Reactivity : Rat
Yang CH et al. Arch Virol 2017; (Epub) Influenza A virus upregulates PRPF8 gene expression to increase virus production.
Chen YH et al. PLoS One 2016; 11 (12):e0168765 Silibinin Inhibits Platelet-Derived Growth Factor-Driven Cell Proliferation via Downregulation of N-Glycosylation in Human Tenon's Fibroblasts in a Proteasome-Dependent Manner.
Application : WB
Reactivity : Human
Thi Do D et al. Cytotechnology 2016; Novel regulations of MEF2-A, MEF2-D, and CACNA1S in the functional incompetence of adipose-derived mesenchymal stem cells by induced indoxyl sulfate in chronic kidney disease.
Application : WB
Liu CH et al. PLoS One 2014; 9 (8):e94995 C1GALT1 promotes invasive phenotypes of hepatocellular carcinoma cells by modulating integrin β1 glycosylation and activity.
Application : WB
Reactivity : Human
Hsu HY et al. Evid Based Complement Alternat Med 2012; 2012:819632 Antimigratory Effects of the Methanol Extract from Momordica charantia on Human Lung Adenocarcinoma CL1 Cells.
Application : WB
Reactivity : Human
SDS
Sodium Azide.pdf
Package List Price ($)
$ 419