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Actin antibody [AC-40]

Anti-Actin antibody [AC-40] used in IHC (Frozen sections) (IHC-Fr). GTX11003
Anti-Actin antibody [AC-40] used in Western Blot (WB). GTX11003
Anti-Actin antibody [AC-40] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11003

Cat No. GTX11003

Host

Mouse

Clonality

Monoclonal

Clone Name

AC-40

Isotype

IgG2a

Application

WB, ICC/IF, IHC-Fr, ELISA

Reactivity

Human, Mouse, Rat, Rabbit, Goat, Sheep, Bovine, Dog, Hamster, Chicken, Guinea pig, Pig, Monkey, Carp, Snail, Viper, Xenopus
Package
100 μl ($389)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF 1:200
IHC-Fr Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

human or chicken fibroblast

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

synthetic actin C-terminal peptide Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe, attached to a Multiple Antigen Peptide (MAP) backbone.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_384201

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

actin alpha 1, skeletal muscle , ACTA , ASMA , CFTD , CFTD1 , CFTDM , MPFD , NEM1 , NEM2 , NEM3 , SHPM

Cellular Localization

Cytoplasm, cytoskeleton

Background

The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.

Database

  • Gene ID: 58 ACTA1
  • UniProt: P68133 ACTA1

Research Area

DATA IMAGES

Anti-Actin antibody [AC-40] used in IHC (Frozen sections) (IHC-Fr). GTX11003

GTX11003 IHC-Fr Image

IHC-Fr analysis of human tongue tissue using GTX11003 Actin antibody [AC-40].

Anti-Actin antibody [AC-40] used in Western Blot (WB). GTX11003

GTX11003 WB Image

WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK, and (9) MDCK lysates using Actin antibody [AC-40] at 1:500.

Anti-Actin antibody [AC-40] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11003

GTX11003 ICC/IF Image

ICC/IF analysis of Rat2 cells using GTX11003 Actin antibody [AC-40] at 1:250 (red) with DAPI (blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.

REFERENCE

REVIEW

Actin antibody [AC-40] Cat No. GTX11003

Rating ( Average 5 based on 6 users reviews)
Application
Western Blot(WB) ( Average 5 based on 6 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Elife in 2019. PMID: 31284899
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019. PMID: 30846715
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019. PMID: 31892709
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Evid Based Complement Alternat Med in 2013. PMID: 23573126
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Stem Cell Res Ther in 2017. PMID: 28482861
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014. PMID: 25089569
Application Reference
SDS
Sodium Azide.pdf
Package List Price ($)
$ 389