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Actin antibody [BA3R]

Anti-Actin antibody [BA3R] used in Western Blot (WB). GTX82559

Cat. No. GTX82559

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 BA3R

Isotype

 IgG2b

Application

 WB, ICC/IF, ELISA, IHC

Reactivity

 Human, Mouse, Rat, Rabbit, Chicken
Package
100 μg ($399)
See all Actin products

APPLICATION

Application Note

Recommended Starting Dilutions:
WB (with ECL): Use at a dilution of 1:1000-10000
Immunostaining: Use at a dilution of 1:500 - 1:2000

For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Product Note

Recognizes native and denatured forms of β-Actin (∼42kDa)

PROPERTIES

Form

Liquid

Buffer

10mM PBS pH7.2

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

β-Actin N-terminal peptide-KLH conjugates

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PS1TP5BP1 , beta cytoskeletal actin , Beta-actin , Betaactin , actin , beta , PS1TP5-binding protein 1 , actin , cytoplasmic1 , actin , cytoplasmic 1 , PS1TP5binding protein1 , PS1TP5binding protein 1 , Beta actin , PS1TP5 binding protein 1

Background

Actin is a ubiquitous globular protein that is one of the most highly-conserved proteins known. It is foundin two main states; G-actin is the globular monomeric form, whereas F-actin forms helical polymers. Both G-and F-actin are intrinsically flexible structures - a feature vital in actin's role as a dynamic filamentnetwork. Actin has four major functions. Firstly, F-actin polymers form microfilaments - polar intracellular'tracks' for kinesin motor proteins, allowing the transport of vesicles, organelles and other cargo. Actinis a component of the cytoskeleton and links to alpha-actinin, E-cadherin and beta-catenin at adherensjunctions. This gives mechanical support to cells and attaches them to each other and the extracellularmatrix. In muscle cells, actin-rich thin filaments associate with myosin-rich thick filaments to formactomyosin myofibrils. Using energy from the hydrolysis of ATP, myofibrils undergo cyclic shortening throughactin-myosin head interactions, which represents the mechanics of muscle contraction. Finally, actin has arole in cell motility through polymerization and depolymerization of fibrils.

Research Area

DATA IMAGES

Anti-Actin antibody [BA3R] used in Western Blot (WB). GTX82559

GTX82559 WB Image

1:1000 (1μg/mL) Ab dilution used in WB of 20μg/lane tissue lysates from human (1), mouse (2), rat (3), and rabbit (4)

REFERENCE

Application Reference

Roc?o Valle-Bautista et al. Front Neurosci 2021; 15 : 740282 Long-Term Functional and Cytoarchitectonic Effects of the Systemic Administration of the Histamine H1 Receptor Antagonist/Inverse Agonist Chlorpheniramine During Gestation in the Rat Offspring Primary Motor Cortex.
Application : WB
Reactivity : Rat
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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations