Actin antibody [HL2372]

Cat. No. GTX638580

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL2372
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat, Rabbit, Drosophila, Cat, Dog, African green monkey
Package	100 μl ($399), 25 μl ($169)

See all Actin products

Datasheet

View product citations for antibody GTX638580 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Actin.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin alpha cardiac muscle 1 , ACTC , ASD5 , CMD1R , CMH11 , LVNC4

Cellular Localization

Cytoplasm, cytoskeleton

Background

Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq, Jul 2008]

Database

Gene ID: 70 ACTC1
UniProt: P68032 ACTC1

Research Area

DATA IMAGES

GTX638580 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Actin antibody [HL2372] (GTX638580) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX638580 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Actin antibody [HL2372] (GTX638580) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX638580 ICC/IF Image

Actin antibody [HL2372] detects Actin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Actin stained by Actin antibody [HL2372] (GTX638580) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).

GTX638580 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Actin antibody [HL2372] (GTX638580) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX638580 IHC-P Image

Actin antibody [HL2372] detects Actin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Actin stained by Actin antibody [HL2372] (GTX638580) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX638580 IHC-P Image

Actin antibody [HL2372] detects Actin protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Actin stained by Actin antibody [HL2372] (GTX638580) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX638580 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Actin antibody (GTX638580) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

Actin antibody [HL2372]

Cat. No. GTX638580

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX638580 WB Image

GTX638580 WB Image

GTX638580 WB Image

GTX638580 ICC/IF Image

GTX638580 WB Image

GTX638580 IHC-P Image

GTX638580 IHC-P Image

GTX638580 WB Image

GTX638580 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

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