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Active Ghrelin antibody

Anti-Active Ghrelin antibody used in ELISA (ELISA). GTX78202

Cat No. GTX78202

Host

Chicken

Clonality

Polyclonal

Isotype

IgY

Application

ELISA

Reactivity

Human, Mouse
Package
200 μg ($379)

APPLICATION

Application Note

For ELISA: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

13 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

GSS(n_octanoyl)FLSPEH

Purification

Affinity purified with antigen

Conjugation

Unconjugated

RRID

AB_625897

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ghrelin and obestatin prepropeptide , MTLRP

Cellular Localization

Secreted

Background

This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES

Anti-Active Ghrelin antibody used in ELISA (ELISA). GTX78202

GTX78202 ELISA Image

GTX78202 in Sandwich ELISA for analysis of active ghrelin. Chicken anti-ghrelin C-terminal peptide (GTX78203) was coated on ELISA plate. Various concentrations of active ghrelin were incubated and detected with biotinylated GTX78202 at a 1:1000 dilution

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 379