GeneTex
United States (US)

Activin Receptor Type IA antibody

GTX107815_40142_20160824_IHC-Fr_19011507_653.jpg
GTX107815_40142_WB_R_liver_19011507_384.jpg
GTX107815_40142_20160811_WB_M_heart_19011507_791.jpg

Cat No. GTX107815

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-Fr
Reactivity Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

mouse heart , rat liver

Predict Reactivity

Human, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Activin Receptor type IA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ACTRI antibody, ACVR1A antibody, ACVRLK2 antibody, ALK2 antibody, FOP antibody, SKR1 antibody, TSRI antibody, ACVR1 antibody, serine/threonine-protein kinase receptor R1 antibody, activin receptor type I antibody, TGF-B superfamily receptor type I antibody, activin receptor-like kinase 2 antibody, hydroxyalkyl-protein kinase antibody, activin A receptor, type II-like kinase 2 antibody, activin receptor type-1 antibody, activin A receptor, type I antibody

Cellular Localization

Membrane

Background

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I ( I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. This gene encodes activin A type I receptor which signals a particular transcriptional response in concert with activin type II receptors. Mutations in this gene are associated with fibrodysplasia ossificans progressive. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX107815_40142_20160824_IHC-Fr_19011507_653.jpg

GTX107815 IHC-Fr Image

Activin A Receptor type I antibody detects Activin A Receptor type I protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Activin A Receptor type I protein stained by Activin A Receptor type I antibody (GTX107815) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107815_40142_WB_R_liver_19011507_384.jpg

GTX107815 WB Image

Activin A Receptor type I antibody detects ACVR1 protein by Western blot analysis.
A. 50 µg rat liver lysate/extract
7.5 % SDS-PAGE
Activin A Receptor type I antibody (GTX107815) dilution: 1:1000

GTX107815_40142_20160811_WB_M_heart_19011507_791.jpg

GTX107815 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Activin A Receptor type I antibody (GTX107815) diluted at 1:500.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf