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Activin Receptor Type IIB antibody [N1C1]

Overexpression
GTX105330_43005_20180126_WB_B_19011506_734.jpg
GTX105330_43005_20180126_WB_M_cerebellum_19011506_250.jpg
GTX105330_43005_20180126_WB_2_19011506_180.jpg
GTX105330_43404_20190118_IHC-P_M_19022001_270.jpg

Cat No. GTX105330

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:50000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR-32 , SK-N-AS , mosue cerebellum , mouse testis , ACVR2B-transfected 293T

Predict Reactivity

Rat, Bovine, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.91mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Activin Receptor Type IIB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ACTRIIB antibody, ActR-IIB antibody, MGC116908 antibody, ACVR2B antibody, activin receptor type-2B antibody, "activin A receptor, type IIB antibody"

Background

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptors are considered to be constitutively active kinases. This gene encodes activin A type IIB receptor, which displays a 3- to 4-fold higher affinity for the ligand than activin A type II receptor. [provided by RefSeq]

Database

DATA IMAGES
GTX105330_43005_20180126_WB_B_19011506_734.jpg

GTX105330 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Activin Receptor Type IIB antibody [N1C1] (GTX105330) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105330_43005_20180126_WB_M_cerebellum_19011506_250.jpg

GTX105330 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Activin Receptor Type IIB antibody [N1C1] (GTX105330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105330_43005_20180126_WB_2_19011506_180.jpg

GTX105330 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Activin Receptor Type IIB antibody [N1C1] (GTX105330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105330_43404_20190118_IHC-P_M_19022001_270.jpg

GTX105330 IHC-P Image

Activin Receptor Type IIB antibody [N1C1] detects Activin Receptor Type IIB protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
Activin Receptor Type IIB stained by Activin Receptor Type IIB antibody [N1C1] (GTX105330) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169