GeneTex
United States (US)

Adenosine A1 Receptor antibody [N3C2], Internal

GTX100025_WB_19011506_754.jpg
GTX100025_WB_M_19011506_786.jpg

Cat No. GTX100025

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , NIH-3T3

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Adenosine A1 Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

RDC7 antibody, ADORA1 antibody, adenosine receptor A1 antibody, adenosine A1 receptor antibody

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100025_WB_19011506_754.jpg

GTX100025 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
10% SDS PAGE
Adenosine receptor A1 antibody
GTX100025 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100025_WB_M_19011506_786.jpg

GTX100025 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3
10% SDS PAGE
GTX100025 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REVIEW

Adenosine A1 Receptor antibody [N3C2], Internal Cat No. GTX100025

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 15-Jul-2016
Score :
Application Tested : WB
Sample Species : Ms
Sample : 3T3-L1 cells
Amount used : 20μg
Blocking : 6% skim milk in TBS, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf