Adenosine A1 Receptor antibody [N3C2], Internal

Name: Adenosine A1 Receptor antibody [N3C2], Internal
Brand: GeneTex
SKU: GTX100025
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Adenosine A1 Receptor antibody [N3C2], Internal used in Western Blot (WB). GTX100025

Cat. No. GTX100025

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human, Mouse, Cat
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX100025 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000

Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

NIH-3T3

Predict Reactivity

Rat, Rabbit, Bovine, Chicken, Guinea pig, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human Adenosine A1 Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240420

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenosine A1 receptor , RDC7

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene. [provided by RefSeq]

Database

Gene ID: 134 ADORA1
UniProt: P30542 ADORA1

Research Area

DATA IMAGES

GTX100025 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [N3C2], Internal (GTX100025) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100025 WB Image

Sample (30 μg of whole cell lysate)
A:NIH-3T3
10% SDS PAGE
GTX100025 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100025 WB Image

PG-4 whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody (GTX100025) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Mohd Nasir NA et al. Eur J Pharmacol 2020; 887 : 173431 Effect of trans-resveratrol on dexamethasone-induced changes in the expression of MMPs by human trabecular meshwork cells: Involvement of adenosine A₁ receptors and NFkB.

Application : WB

Reactivity : Human

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REVIEW