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Adenosine A1 Receptor antibody

Anti-Adenosine A1 Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX23460
Anti-Adenosine A1 Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23460
Anti-Adenosine A1 Receptor antibody used in Western Blot (WB). GTX23460

Cat. No. GTX23460

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Adenosine A1 Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 2μg/ml
IHC-P 1:50-1:200
FACS 3-5 μg/10⁶ cells
IHC Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Product Note

This antibody does not detect other AR subtypes.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic peptide corresponding to residues C(309) Q P K P P I D E D L P E E K A E D(326) of rat Adenosine Receptor A1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_385060

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenosine A1 receptor

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

inhibits adenylyl cyclase activity; plays a role in regulation of arterial pressure, heart rate, and sodium excretion [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-Adenosine A1 Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX23460

GTX23460 IHC-P Image

IHC-P analysis of rat brain tissue using GTX23460 Adenosine A1 Receptor antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:100

Anti-Adenosine A1 Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23460

GTX23460 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX23460 Adenosine A1 Receptor antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Anti-Adenosine A1 Receptor antibody used in Western Blot (WB). GTX23460

GTX23460 WB Image

WB analysis of whole cell extracts (30 μg lysate) of PC 12 (Lane 1), SH-SY5Y (lane 2), U-87MG (lane 3), A549 (Lane 4) and K-562 (lane 5) using GTX23460 Adenosine A1 Receptor antibody.
Dilution : 1:250-1:1000

REFERENCE

Application Reference

Bairam A et al. Brain Res 2009; 1287 : 74-83 Altered expression of adenosine A1 and A2A receptors in the carotid body and nucleus tractus solitarius of adult male and female rats following neonatal caffeine treatment.
Application : WB
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399