Adenosine A2a Receptor antibody [7F6-G5-A2]

Name: Adenosine A2a Receptor antibody [7F6-G5-A2]
Brand: GeneTex
SKU: GTX04561
Price: 499.00 USD

Anti-Adenosine A2a Receptor antibody [7F6-G5-A2] used in IHC (Paraffin sections) (IHC-P). GTX04561

Anti-Adenosine A2a Receptor antibody [7F6-G5-A2] used in Flow cytometry (FACS). GTX04561

Anti-Adenosine A2a Receptor antibody [7F6-G5-A2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04561

Anti-Adenosine A2a Receptor antibody [7F6-G5-A2] used in Western Blot (WB). GTX04561

Cat. No. GTX04561

Host	Mouse
Clonality	Monoclonal
Clone Name	7F6-G5-A2
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, CyTOF
Reactivity	Human, Mouse, Rat, Rabbit, Sheep, Dog, Guinea pig, Pig
Package	100 μl ($499)

See all Adenosine A2a Receptor products

Datasheet

View product citations for antibody GTX04561 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-P	Assay dependent
IHC-Fr	Assay dependent
FACS	Assay dependent
CyTOF	Assay dependent

Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length recombinant human Adenosine A2a R(UniProt# P29274). Epitope mapped to SQPLPGER in the third intracellular loop (PMID 9822147).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenosine A2a receptor , A2aR , ADORA2 , RDC8

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013]

Database

Gene ID: 135 ADORA2A
UniProt: P29274 ADORA2A

Research Area

DATA IMAGES

GTX04561 IHC-P Image

IHC-P analysis of human brain (putamen) tissue using GTX04561 Adenosine A2a Receptor antibody [7F6-G5-A2].
Dilution : 5 μg/mL

GTX04561 FACS Image

FACS analysis of 4% PFA fixed U-937 cells using GTX04561 Adenosine A2a Receptor antibody [7F6-G5-A2].
Blue : Primary antibody
Orange : Isotype control
Permeabilization : 0.1% saponin
Dilution : 1 μg/mL

GTX04561 ICC/IF Image

ICC/IF analysis of 4% PFA fixed Neuro2a cells using GTX04561 Adenosine A2a Receptor antibody [7F6-G5-A2].
Green : Primary antibody
Red : Actin
Blue : DAPI
Permeabilization : 0.5% Triton X-100
Dilution : 1:50

GTX04561 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX04561 Adenosine A2a Receptor antibody [7F6-G5-A2].
Dilution : 1:400

GTX04561 FACS Image

FACS analysis of 4% PFA fixed human PBMCs cells using GTX04561 Adenosine A2a Receptor antibody [7F6-G5-A2].
Blue : Primary antibody
Orange : Isotype control
Permeabilization : 0.1% saponin
Dilution : 1 μg/mL

GTX04561 FACS Image

FACS analysis of U-87 MG cells using GTX04561 Adenosine A2a Receptor antibody [7F6-G5-A2].
Blue : Primary antibody
Orange : Isotype control
Dilution : 10 μg/mL

GTX04561 WB Image

WB analysis of mouse brain tissue lysates using GTX04561 Adenosine A2a Receptor antibody [7F6-G5-A2].
Dilution : 1 μg/mL

GTX04561 FACS Image

FACS analysis of 4% PFA fixed SH-SY5Y cells using GTX04561 Adenosine A2a Receptor antibody [7F6-G5-A2].
Blue : Primary antibody
Orange : Isotype control
Permeabilization : 0.1% saponin
Dilution : 1 μg/mL

GTX04561 ICC/IF Image

ICC/IF analysis of 4% PFA fixed HeLa cells using GTX04561 Adenosine A2a Receptor antibody [7F6-G5-A2].
Green : Primary antibody
Red : Actin
Blue : DAPI
Permeabilization : 0.5% Triton X-100
Dilution : 1:50

GTX04561 FACS Image

GTX04561 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX04561 Adenosine A2a Receptor antibody [7F6-G5-A2].
Dilution : 1:100

GTX04561 FACS Image

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 499

Qty

Adenosine A2a Receptor antibody [7F6-G5-A2]

Cat. No. GTX04561

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX04561 IHC-P Image

GTX04561 FACS Image

GTX04561 ICC/IF Image

GTX04561 IHC-P Image

GTX04561 FACS Image

GTX04561 FACS Image

GTX04561 WB Image

GTX04561 FACS Image

GTX04561 FACS Image

GTX04561 ICC/IF Image

GTX04561 FACS Image

GTX04561 IHC-P Image

GTX04561 FACS Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.