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Adenosine kinase antibody [N1C1]

KO/KD
Anti-Adenosine kinase antibody [N1C1] used in Western Blot (WB). GTX101372
Anti-Adenosine kinase antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101372
Anti-Adenosine kinase antibody [N1C1] used in Western Blot (WB). GTX101372

Cat. No. GTX101372

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($419),
25 μl ($169)
See all Adenosine kinase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

A431 , HeLa

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Adenosine kinase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036177

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenosine kinase , AK

Background

This gene encodes adenosine kinase, an abundant enzyme in mammalian tissues. The enzyme catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Alternative splicing results in two transcript variants encoding different isoforms. Both isoforms of the enzyme phosphorylate adenosine with identical kinetics and both require Mg2+ for activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Adenosine kinase antibody [N1C1] used in Western Blot (WB). GTX101372

GTX101372 WB Image

KO/KD

Wild-type (WT) and Adenosine kinase knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody [N1C1] (GTX101372) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Adenosine kinase antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101372

GTX101372 ICC/IF Image

Adenosine kinase antibody [N1C1] detects Adenosine kinase protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Adenosine kinase protein stained by Adenosine kinase antibody [N1C1] (GTX101372) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Adenosine kinase antibody [N1C1] used in Western Blot (WB). GTX101372

GTX101372 WB Image

Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
GTX101372 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169