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Adenosine kinase antibody

KO/KD
Anti-Adenosine kinase antibody used in Western Blot (WB). GTX101385
Anti-Adenosine kinase antibody used in Western Blot (WB). GTX101385
Anti-Adenosine kinase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101385
Anti-Adenosine kinase antibody used in Western Blot (WB). GTX101385
Anti-Adenosine kinase antibody used in IHC (Paraffin sections) (IHC-P). GTX101385
Anti-Adenosine kinase antibody used in IHC (Paraffin sections) (IHC-P). GTX101385
Anti-Adenosine kinase antibody used in IHC (Paraffin sections) (IHC-P). GTX101385

Cat. No. GTX101385

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Adenosine kinase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

mouse liver , rat liver

Predict Reactivity

Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Adenosine kinase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036178

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenosine kinase , AK

Background

This gene encodes adenosine kinase, an abundant enzyme in mammalian tissues. The enzyme catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Alternative splicing results in two transcript variants encoding different isoforms. Both isoforms of the enzyme phosphorylate adenosine with identical kinetics and both require Mg2+ for activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Adenosine kinase antibody used in Western Blot (WB). GTX101385

GTX101385 WB Image

KO/KD

Wild-type (WT) and Adenosine kinase knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody (GTX101385) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Adenosine kinase antibody used in Western Blot (WB). GTX101385

GTX101385 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody (GTX101385) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Adenosine kinase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101385

GTX101385 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Adenosine kinase(GTX101385) antibody at 1:200 dilution.

Anti-Adenosine kinase antibody used in Western Blot (WB). GTX101385

GTX101385 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody (GTX101385) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Adenosine kinase antibody used in IHC (Paraffin sections) (IHC-P). GTX101385

GTX101385 IHC-P Image

Adenosine kinase antibody detects ADK protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ADK stained by Adenosine kinase antibody (GTX101385) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Adenosine kinase antibody used in IHC (Paraffin sections) (IHC-P). GTX101385

GTX101385 IHC-P Image

Adenosine kinase antibody detects Adenosine kinase protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Adenosine kinase stained by Adenosine kinase antibody (GTX101385) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Adenosine kinase antibody used in IHC (Paraffin sections) (IHC-P). GTX101385

GTX101385 IHC-P Image

Adenosine kinase antibody detects Adenosine kinase protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Adenosine kinase stained by Adenosine kinase antibody (GTX101385) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Yang Y et al. Nat Metab 2020; 2 (4) : 364-379 NRH salvage and conversion to NAD + requires NRH kinase activity by adenosine kinase.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169