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Adenylate Kinase 4 antibody

Anti-Adenylate Kinase 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX49379
Anti-Adenylate Kinase 4 antibody used in Western Blot (WB). GTX49379
Anti-Adenylate Kinase 4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49379

Cat No. GTX49379

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

25 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of Adenylate Kinase 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Adenylate Kinase 4,Ak 4,Ak3,Ak3L1,Ak3L2,Ak4

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Adenylate Kinase 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX49379

GTX49379 IHC-P Image

IHC-P analysis of formalin fixed rat kidney tissue section using GTX49379 Adenylate Kinase 4 antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-Adenylate Kinase 4 antibody used in Western Blot (WB). GTX49379

GTX49379 WB Image

WB analysis of HepG2 (A), Raji (B), mouse liver (C), mouse kidney (D) whole cell lysates using GTX49379 Adenylate Kinase 4 antibody.

Anti-Adenylate Kinase 4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49379

GTX49379 ICC/IF Image

ICC/IF analysis of formalin-fixed A549 cells using GTX49379 Adenylate Kinase 4 antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

REFERENCE
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REVIEW
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Package List Price ($)
$ 319