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Adenylate Kinase 4 antibody

Anti-Adenylate Kinase 4 antibody used in Western Blot (WB). GTX65835
Anti-Adenylate Kinase 4 antibody used in Immunoprecipitation (IP). GTX65835
Anti-Adenylate Kinase 4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65835

Cat No. GTX65835

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Mouse
Package
100 μl ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

25 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-223 of human AK4 (NP_037542.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Adenylate Kinase 4,Ak 4,Ak3,Ak3L1,Ak3L2,Ak4

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq, Jul 2008]

Research Area

DATA IMAGES
Anti-Adenylate Kinase 4 antibody used in Western Blot (WB). GTX65835

GTX65835 WB Image

WB analysis of extracts of various cell lines using GTX65835 Adenylate Kinase 4 antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-Adenylate Kinase 4 antibody used in Immunoprecipitation (IP). GTX65835

GTX65835 IP Image

IP analysis of HepG2 cells using GTX65835 Adenylate Kinase 4 antibody.
IP reaction : 0.8μl antibody/200μg lysate
Dilution : 1:1000

Anti-Adenylate Kinase 4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65835

GTX65835 ICC/IF Image

ICC/IF analysis of A549 cells using GTX65835 Adenylate Kinase 4 antibody.
Blue : DAPI

REFERENCE
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REVIEW
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Package List Price ($)
$ 329