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Adenylate kinase 1 antibody [8A1]

KO/KD Overexpression
Anti-Adenylate kinase 1 antibody [8A1] used in Flow cytometry (FACS). GTX84938
Anti-Adenylate kinase 1 antibody [8A1] used in Western Blot (WB). GTX84938
Anti-Adenylate kinase 1 antibody [8A1] used in Western Blot (WB). GTX84938
Anti-Adenylate kinase 1 antibody [8A1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84938
Anti-Adenylate kinase 1 antibody [8A1] used in Western Blot (WB). GTX84938

Cat. No. GTX84938

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8A1

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Monkey
Package
100 μl ($519)
See all Adenylate kinase 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF 1:100
IHC-P Assay dependent
FACS 1:100
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human AK1 (NP_000467) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_10729931

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenylate kinase 1 , HTL-S-58j

Cellular Localization

Cytoplasm

Background

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth.

Database

Research Area

DATA IMAGES

Anti-Adenylate kinase 1 antibody [8A1] used in Flow cytometry (FACS). GTX84938

GTX84938 FACS Image

FACS analysis of HEK293T cells transfected with either Adenylate kinase 1 plasmid(Red) or empty vector control plasmid(Blue) using GTX84938 Adenylate kinase 1 antibody [8A1].

Anti-Adenylate kinase 1 antibody [8A1] used in Western Blot (WB). GTX84938

GTX84938 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Adenylate kinase 1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84938 Adenylate kinase 1 antibody [8A1].
Loading : 5 ug per lane

Anti-Adenylate kinase 1 antibody [8A1] used in Western Blot (WB). GTX84938

GTX84938 WB Image

WB analysis of various cell lines using GTX84938 Adenylate kinase 1 antibody [8A1].
Loading : 35 ug per lane

Anti-Adenylate kinase 1 antibody [8A1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84938

GTX84938 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Adenylate kinase 1 plasmid using GTX84938 Adenylate kinase 1 antibody [8A1].

Anti-Adenylate kinase 1 antibody [8A1] used in Western Blot (WB). GTX84938

GTX84938 WB Image

KO/KD

WB analysis of wild type and Adenylate kinase 1 knock out HeLa cell lysate(10μg per lane) using GTX84938 Adenylate kinase 1 antibody [8A1].
Dilution : 1:500

REFERENCE

Application Reference

Jan YH et al. Sci Rep 2019; 9 (1) : 12329 A co-expressed gene status of adenylate kinase 1/4 reveals prognostic gene signature associated with prognosis and sensitivity to EGFR targeted therapy in lung adenocarcinoma.
Application : WB, IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519