GeneTex
United States (US)

Adenylate kinase 1 antibody [N1C3]

GTX106079_39890_WB_M_adipose_19011506_658.jpg
GTX106079_39890_IHC_19011506_297.jpg
GTX106079_WB_19011506_846.jpg

Cat No. GTX106079

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

A549 , HCT116 , mouse brown adipose tissue

Predict Reactivity

Rat, Sheep, Bovine, Dog, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Adenylate kinase 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AK1 antibody, ATP-AMP transphosphorylase 1 antibody, adenylate kinase isoenzyme 1 antibody, AK 1 antibody, myokinase antibody, adenylate kinase 1 antibody

Cellular Localization

Cytoplasm

Background

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106079_39890_WB_M_adipose_19011506_658.jpg

GTX106079 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brown adipose tissue
12% SDS PAGE
GTX106079 diluted at 1:1000

GTX106079_39890_IHC_19011506_297.jpg

GTX106079 IHC-P Image

Adenylate kinase 1 antibody [N1C3] detects Adenylate kinase 1 protein at cytosol on Ca922 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Ca922 xenograft.
Adenylate kinase 1 antibody [N1C3] (GTX106079) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106079_WB_19011506_846.jpg

GTX106079 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116 12% SDS PAGE
GTX106079 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169