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Adenylate kinase 1 antibody [N1C3]

KO/KD
Anti-Adenylate kinase 1 antibody [N1C3] used in Western Blot (WB). GTX106079
Anti-Adenylate kinase 1 antibody [N1C3] used in Western Blot (WB). GTX106079
Anti-Adenylate kinase 1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106079

Cat. No. GTX106079

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Adenylate kinase 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

Mouse brown adipose tissue

Predict Reactivity

Rat, Sheep, Bovine, Dog, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Adenylate kinase 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036192

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenylate kinase 1 , HTL-S-58j

Cellular Localization

Cytoplasm

Background

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Adenylate kinase 1 antibody [N1C3] used in Western Blot (WB). GTX106079

GTX106079 WB Image

KO/KD

Wild-type (WT) and Adenylate kinase 1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Adenylate kinase 1 antibody [N1C3] (GTX106079) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Adenylate kinase 1 antibody [N1C3] used in Western Blot (WB). GTX106079

GTX106079 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brown adipose tissue
12% SDS PAGE
GTX106079 diluted at 1:1000

Anti-Adenylate kinase 1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106079

GTX106079 IHC-P Image

Adenylate kinase 1 antibody [N1C3] detects Adenylate kinase 1 protein at cytosol on Ca922 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Ca922 xenograft.
Adenylate kinase 1 antibody [N1C3] (GTX106079) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169