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Adenylate kinase 2 antibody [N1C2]

KO/KD
Anti-Adenylate kinase 2 antibody [N1C2] used in Western Blot (WB). GTX111726
Anti-Adenylate kinase 2 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111726
Anti-Adenylate kinase 2 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX111726
Anti-Adenylate kinase 2 antibody [N1C2] used in Western Blot (WB). GTX111726
Anti-Adenylate kinase 2 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX111726

Cat. No. GTX111726

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all Adenylate kinase 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

H1299 , HepG2 , 293T

Predict Reactivity

Mouse, Zebrafish, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Adenylate kinase 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949612

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenylate kinase 2 , ADK2

Cellular Localization

Mitochondrion intermembrane space

Background

Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Adenylate kinase 2 antibody [N1C2] used in Western Blot (WB). GTX111726

GTX111726 WB Image

KO/KD

Wild-type (WT) and Adenylate kinase 2 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Adenylate kinase 2 antibody (GTX111726) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Adenylate kinase 2 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111726

GTX111726 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Adenylate kinase 2(GTX111726) antibody at 1:200 dilution.

Anti-Adenylate kinase 2 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX111726

GTX111726 IHC-P Image

Adenylate kinase 2 antibody [N1C2] detects Adenylate kinase 2 protein at mitochondria in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Adenylate kinase 2 antibody [N1C2] (GTX111726) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Adenylate kinase 2 antibody [N1C2] used in Western Blot (WB). GTX111726

GTX111726 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hep G2 (GTX27900)
12% SDS PAGE
GTX111726 diluted at 1:1000

Anti-Adenylate kinase 2 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX111726

GTX111726 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Adenylate kinase 2(GTX111726) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169