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Adenylate kinase 4 antibody [N1C3-2]

Anti-Adenylate kinase 4 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX107482
Anti-Adenylate kinase 4 antibody [N1C3-2] used in Western Blot (WB). GTX107482
Anti-Adenylate kinase 4 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX107482
Anti-Adenylate kinase 4 antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107482
Anti-Adenylate kinase 4 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX107482

Cat. No. GTX107482

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Adenylate kinase 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

HeLa , HepG2 , HCT116

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Adenylate kinase 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036193

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenylate kinase 4 , AK 4 , AK3 , AK3L1 , AK3L2

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Adenylate kinase 4 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX107482

GTX107482 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Adenylate kinase 4(GTX107482) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Adenylate kinase 4 antibody [N1C3-2] used in Western Blot (WB). GTX107482

GTX107482 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
B: HepG2
C: HCT116
12% SDS PAGE
GTX107482 diluted at 1:1000

Anti-Adenylate kinase 4 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX107482

GTX107482 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using Adenylate kinase 4(GTX107482) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Adenylate kinase 4 antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107482

GTX107482 ICC/IF Image

Adenylate kinase 4 antibody [N1C3-2] detects Adenylate kinase 4 protein at mitochondria by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: Adenylate kinase 4 protein stained by Adenylate kinase 4 antibody [N1C3-2] (GTX107482) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Adenylate kinase 4 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX107482

GTX107482 IHC-P Image

Adenylate kinase 4 antibody [N1C3-2] detects Adenylate kinase 4 protein at mitochondria in mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Adenylate kinase 4 antibody [N1C3-2] (GTX107482) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Jan YH et al. Sci Rep 2019; 9 (1) : 12329 A co-expressed gene status of adenylate kinase 1/4 reveals prognostic gene signature associated with prognosis and sensitivity to EGFR targeted therapy in lung adenocarcinoma.
Application : WB, IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169