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Adenylate kinase 4 antibody [N1C3]

Anti-Adenylate kinase 4 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104430
Anti-Adenylate kinase 4 antibody [N1C3] used in Western Blot (WB). GTX104430
Anti-Adenylate kinase 4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104430

Cat. No. GTX104430

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Adenylate kinase 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Raji

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Adenylate kinase 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949615

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenylate kinase 4 , AK 4 , AK3 , AK3L1 , AK3L2

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Adenylate kinase 4 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104430

GTX104430 ICC/IF Image

Adenylate kinase 4 antibody [N1C3] detects Adenylate kinase 4 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: Adenylate kinase 4 protein stained by Adenylate kinase 4 antibody [N1C3] (GTX104430) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Adenylate kinase 4 antibody [N1C3] used in Western Blot (WB). GTX104430

GTX104430 WB Image

Sample(30 ug whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: Hep G2 (GTX27900)
F: Raji (GTX27908)
12% SDS PAGE
GTX104430 diluted at 1:1000

Anti-Adenylate kinase 4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104430

GTX104430 IHC-P Image

Immunohistochemical analysis of paraffin-embedded gastric cancer N87 xenograft, using Adenylate kinase 4(GTX104430) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Jan YH et al. Cancer Res 2012; 72 (19) : 5119-29 Adenylate kinase-4 is a marker of poor clinical outcomes that promotes metastasis of lung cancer by downregulating the transcription factor ATF3.
Application : IHC
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169