GeneTex
United States (US)

Adiponectin Receptor 1 antibody [C2C3], C-term

GTX104770_40212_WB_R_Muscle_19011506_874.jpg
GTX104770_39673_WB_M_White adipose tissue_19011720_475.jpg
GTX104770_41843_WB_19011506_638.jpg
GTX104770_41843_WB_M_muscle_19011506_498.jpg
GTX104770_39673_IHC_M_19011506_482.jpg

Cat No. GTX104770

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Observed MW

37 kDa. The observed M.W. is based on the following publication. PMID: 21289205

Positive Control

MCF-7 , Mouse white adipose tissue , mouse muscle , rat muscle

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Adiponectin Receptor 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Adiponectin Receptor 1,Acdcr1,Cgi-45,Cgi45,Paqr1,Tesbp1A,Adipor1

Cellular Localization

Membrane

Background

The adiponectin receptors, ADIPOR1 and ADIPOR2 (MIM 607946), serve as receptors for globular and full-length adiponectin (MIM 605441) and mediate increased AMPK (see MIM 602739) and PPAR-alpha (PPARA; MIM 170998) ligand activities, as well as fatty acid oxidation and glucose uptake by adiponectin (Yamauchi et al., 2003 [PubMed 12802337]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX104770_40212_WB_R_Muscle_19011506_874.jpg

GTX104770 WB Image

Sample (50 µg of whole cell lysate)
A: rat muscle
10% SDS PAGE
GTX104770 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104770_39673_WB_M_White adipose tissue_19011720_475.jpg

GTX104770 WB Image

Sample (50 µg of whole cell lysate)
A: mouse white adipose tissue
10% SDS PAGE
GTX104770 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104770_41843_WB_19011506_638.jpg

GTX104770 WB Image

Adiponectin Receptor 1 antibody detects Adiponectin Receptor 1 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Adiponectin Receptor 1 antibody (GTX104770) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104770_41843_WB_M_muscle_19011506_498.jpg

GTX104770 WB Image

Adiponectin Receptor 1 antibody detects Adiponectin Receptor 1 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Adiponectin Receptor 1 antibody (GTX104770) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104770_39673_IHC_M_19011506_482.jpg

GTX104770 IHC-P Image

Adiponectin Receptor 1 antibody [C2C3], C-term detects Adiponectin Receptor 1 protein at membrane on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Adiponectin Receptor 1 antibody [C2C3], C-term (GTX1
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169