GeneTex

  • Country / Location Selection

United States (US)

Adiponectin Receptor 1 antibody [C2C3], C-term

Orthogonal
Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104770
Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in Western Blot (WB). GTX104770
Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in Western Blot (WB). GTX104770
Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in Western Blot (WB). GTX104770
Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in Western Blot (WB). GTX104770
Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in Western Blot (WB). GTX104770
Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in Western Blot (WB). GTX104770

Cat. No. GTX104770

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Adiponectin Receptor 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Observed MW

37 kDa. The observed M.W. is based on the following publication. PMID: 21289205

Positive Control

Mouse white adipose tissue , rat muscle , mouse muscle

Predict Reactivity

Sheep, Bovine, Cat, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human Adiponectin Receptor 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240425

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adiponectin receptor 1 , ACDCR1 , CGI-45 , CGI45 , PAQR1 , TESBP1A

Cellular Localization

Membrane

Background

The adiponectin receptors, ADIPOR1 and ADIPOR2 (MIM 607946), serve as receptors for globular and full-length adiponectin (MIM 605441) and mediate increased AMPK (see MIM 602739) and PPAR-alpha (PPARA; MIM 170998) ligand activities, as well as fatty acid oxidation and glucose uptake by adiponectin (Yamauchi et al., 2003 [PubMed 12802337]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104770

GTX104770 IHC-P Image

Adiponectin Receptor 1 antibody [C2C3], C-term detects Adiponectin Receptor 1 protein at membrane on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Adiponectin Receptor 1 antibody [C2C3], C-term (GTX104770) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in Western Blot (WB). GTX104770

GTX104770 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Adiponectin Receptor 1 antibody [C2C3], C-term (GTX104770) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in Western Blot (WB). GTX104770

GTX104770 WB Image

Sample (50 μg of whole cell lysate)
A: mouse white adipose tissue
10% SDS PAGE
GTX104770 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in Western Blot (WB). GTX104770

GTX104770 WB Image

Sample (50 μg of whole cell lysate)
A: rat muscle
10% SDS PAGE
GTX104770 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in Western Blot (WB). GTX104770

GTX104770 WB Image

Adiponectin Receptor 1 antibody detects Adiponectin Receptor 1 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Adiponectin Receptor 1 antibody (GTX104770) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in Western Blot (WB). GTX104770

GTX104770 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Adiponectin Receptor 1 antibody [C2C3], C-term (GTX104770) diluted at 1:500.

Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in Western Blot (WB). GTX104770

GTX104770 WB Image

Orthogonal

The data was published in the journal PLoS One in 2018.PMID: 30308063

REFERENCE

Application Reference

Li HF et al. iScience 2022; 25 (12) : 105631 Role of PVAT in obesity-related cardiovascular disease through the buffering activity of ATF3.
Ito R et al. PLoS One 2018; 13 (10) : e0205645 Activation of adiponectin receptors has negative impact on * in C2C12 myotubes and fast-type mouse skeletal muscle.
Application : WB
Reactivity : Mouse
Takahashi Y et al. Metabolism 2018; (Epub) Novel phytopeptide osmotin mimics preventive effects of adiponectin on vascular inflammation and atherosclerosis.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Adiponectin Receptor 1 antibody [C2C3], C-term (GTX104770). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169