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Adiponectin Receptor 1 antibody [C2C3], C-term

Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in Western Blot (WB). GTX104770
Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in Western Blot (WB). GTX104770
Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in Western Blot (WB). GTX104770
Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in Western Blot (WB). GTX104770
Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104770

Cat No. GTX104770

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Observed MW

37 kDa. The observed M.W. is based on the following publication. PMID: 21289205

Positive Control

MCF-7 , Mouse white adipose tissue , mouse muscle , rat muscle

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Adiponectin Receptor 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240425

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

adiponectin receptor 1 , ACDCR1 , CGI-45 , CGI45 , PAQR1 , TESBP1A

Cellular Localization

Membrane

Background

The adiponectin receptors, ADIPOR1 and ADIPOR2 (MIM 607946), serve as receptors for globular and full-length adiponectin (MIM 605441) and mediate increased AMPK (see MIM 602739) and PPAR-alpha (PPARA; MIM 170998) ligand activities, as well as fatty acid oxidation and glucose uptake by adiponectin (Yamauchi et al., 2003 [PubMed 12802337]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in Western Blot (WB). GTX104770

GTX104770 WB Image

Sample (50 μg of whole cell lysate)
A: rat muscle
10% SDS PAGE
GTX104770 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in Western Blot (WB). GTX104770

GTX104770 WB Image

Sample (50 μg of whole cell lysate)
A: mouse white adipose tissue
10% SDS PAGE
GTX104770 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in Western Blot (WB). GTX104770

GTX104770 WB Image

Adiponectin Receptor 1 antibody detects Adiponectin Receptor 1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Adiponectin Receptor 1 antibody (GTX104770) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in Western Blot (WB). GTX104770

GTX104770 WB Image

Adiponectin Receptor 1 antibody detects Adiponectin Receptor 1 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Adiponectin Receptor 1 antibody (GTX104770) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Adiponectin Receptor 1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104770

GTX104770 IHC-P Image

Adiponectin Receptor 1 antibody [C2C3], C-term detects Adiponectin Receptor 1 protein at membrane on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Adiponectin Receptor 1 antibody [C2C3], C-term (GTX104770) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

Adiponectin Receptor 1 antibody [C2C3], C-term Cat No. GTX104770

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2018.PMID: 30308063
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf