GeneTex

  • Country / Location Selection

United States (US)

Adiponectin antibody [19F1]

Orthogonal
Anti-Adiponectin antibody [19F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80683
Anti-Adiponectin antibody [19F1] used in Western Blot (WB). GTX80683
Anti-Adiponectin antibody [19F1] used in IHC (Paraffin sections) (IHC-P). GTX80683
Anti-Adiponectin antibody [19F1] used in Western Blot (WB). GTX80683
Anti-Adiponectin antibody [19F1] used in IHC (Paraffin sections) (IHC-P). GTX80683

Cat. No. GTX80683

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 19F1

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA, IHC

Reactivity

 Human, Mouse, Rabbit
Package
100 μg ($399)
See all Adiponectin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/ml
ICC/IF 2-4 μg/ml
IHC-P Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length, recombinant, human adiponectin.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adiponectin, C1Q and collagen domain containing , ACDC , ACRP30 , ADIPQTL1 , ADPN , APM-1 , APM1 , GBP28

Cellular Localization

Secreted

Background

This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010]

Database

Research Area

DATA IMAGES

Anti-Adiponectin antibody [19F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80683

GTX80683 ICC/IF Image

Orthogonal

ICC/IF analysis of differentiated 3T3-L1 cells (treated with StemPro Adipogenesis Supplement for 5 days) using GTX80683 Adiponectin antibody [19F1]. Panel e is untreated cell with less signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA incubated for 3 hours at room temperature

Anti-Adiponectin antibody [19F1] used in Western Blot (WB). GTX80683

GTX80683 WB Image

WB analysis of rabbit subcutaneous white adipose tissue (scWAT), gonadal white adipose tissue (gWAT), perirenal white adipose tissue (pWAT), tibia marrow adipose tissue (tibMAT), tibia red marrow (tibRM), and 3T3-L1 positive control adipocyte lysates using GTX80683 Adiponectin antibody [19F1].
Dilution : 1:1000

Anti-Adiponectin antibody [19F1] used in IHC (Paraffin sections) (IHC-P). GTX80683

GTX80683 IHC-P Image

IHC-P analysis of human skin tissue using GTX80683 Adiponectin antibody [19F1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-Adiponectin antibody [19F1] used in Western Blot (WB). GTX80683

GTX80683 WB Image

WB analysis of 10 μl of conditioned media of HepG2 cell using GTX80683 Adiponectin antibody [19F1].
Dilution : 2 μg/ml

Anti-Adiponectin antibody [19F1] used in IHC (Paraffin sections) (IHC-P). GTX80683

GTX80683 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX80683 Adiponectin antibody [19F1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

REFERENCE

Application Reference

Mann JP et al. Elife 2023; 12 A mouse model of human mitofusin-2-related lipodystrophy exhibits adipose-specific mitochondrial stress and reduced leptin secretion.
Corsetti G et al. Nutrients 2022; 14 (14) Essential Amino Acids-Rich Diet Decreased Adipose Tissue Storage in Adult Mice: A Preliminary Histopathological Study.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for Adiponectin antibody [19F1] (GTX80683). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399