GeneTex
United States (US)

Adiponectin antibody [Ne.Na]

Acrp30-antibody-NeNa_WB_GTX16086-1_18121409_255.jpg

Cat No. GTX16086

Host Mouse
Clonality Monoclonal
Clone Name Ne.Na
Isotype IgG1
Application WB, IP, ELISA
Reactivity Human
APPLICATION

Application Note

ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 μg/ml. Predicted molecular weight: 29 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

26 kDa. ( Note )

Specificity/Sensitivity

Recognizes human ACRP30headless (tail of adiponectin). Does not cross-react with full length adiponectin.
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline containing 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human ACRP30 headless (tail adiponectin) protein.

Purification

>95% by SDS-PAGE

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Adiponectin, C1Q And Collagen Domain Containing,Acdc,Acrp30,Adipqtl1,Adpn,Apm-1,Apm1,Gbp28,Adipoq

Cellular Localization

Secreted

Background

Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase, leptin, and adipocyte complement related protein of 30 kDa (Acrp30). Acrp30 is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, mRNA levels of Acrp30 have been shown to be induced over 100-fold. Studies indicate that Acrp30 enhances the ability of sub-physiological levels of insulin to suppress glucose production, thus linking adipose tissue to whole body glucose regulation. Acrp30 function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Acrp30 secretion is blocked by pharmacologic inhibitors of this kinase. mRNA levels of Acrp30 have been shown to be significantly reduced in adipose tissue of obese patients with Type 2 diabetes relative to control subjects. Structural similarities to TNF alpha suggests that Acrp30 may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Because Acrp30 is down-regulated in various forms of obesity and its structural similarity to TNF alpha, it is currently being investigated as an important regulator of whole body energy homeostasis.

Database

Research Area

DATA IMAGES
Acrp30-antibody-NeNa_WB_GTX16086-1_18121409_255.jpg

GTX16086 WB Image

Monoclonal antibody to adiponectin (GTX16086) detects human ACRP30headless (tail of adiponectin) by Western blot

Package List Price ($)
$ 319