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Adiponectin antibody

Anti-Adiponectin antibody used in Western Blot (WB). GTX112777
Anti-Adiponectin antibody used in Western Blot (WB). GTX112777
Anti-Adiponectin antibody used in Western Blot (WB). GTX112777
Anti-Adiponectin antibody used in Western Blot (WB). GTX112777
Anti-Adiponectin antibody used in IHC (Paraffin sections) (IHC-P). GTX112777

Cat No. GTX112777

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat, Plant
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

Human plasma , Mouse plasma , rat plasma

Predict Reactivity

Rabbit, Sheep, Bovine, Cat, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Adiponectin Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885323

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

adiponectin, C1Q and collagen domain containing , ACDC , ACRP30 , ADIPQTL1 , ADPN , APM-1 , APM1 , GBP28

Cellular Localization

Secreted

Background

This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Adiponectin antibody used in Western Blot (WB). GTX112777

GTX112777 WB Image

Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Adiponectin antibody (GTX112777) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)

Anti-Adiponectin antibody used in Western Blot (WB). GTX112777

GTX112777 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Adiponectin antibody (GTX112777) diluted at 1:500.

Anti-Adiponectin antibody used in Western Blot (WB). GTX112777

GTX112777 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Adiponectin antibody (GTX112777) diluted at 1:500.

Anti-Adiponectin antibody used in Western Blot (WB). GTX112777

GTX112777 WB Image

Adiponectin antibody detects Adiponectin protein by western blot analysis. Human tissue extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Adiponectin antibody (GTX112777) diluted at 1:500.

Anti-Adiponectin antibody used in IHC (Paraffin sections) (IHC-P). GTX112777

GTX112777 IHC-P Image

Adiponectin antibody detects Adiponectin protein at cytoplasm and secreted by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
Adiponectin stained by Adiponectin antibody (GTX112777) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

Adiponectin antibody Cat No. GTX112777

Rating ( Average 4.5 based on 4 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 4 users reviews)
Date : Anonymous submitted on 27-Dec-2020
Score :
Application Tested : WB
Sample Species : Rat
Sample : Rat plasma
Amount used : 5 μg
Blocking : 2% skimmed milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Date : Anonymous submitted on 31-May-2018
Score :
Application Tested : WB
Sample Species : Ms
Sample : 3T3-L1 cells
Amount used : 50 μg
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 23-Dec-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : white preadipocytes cells
Amount used : 10μg
Blocking : 5% w/v nonfat milk in TBST
Primary Antibody : 1:1000, 25°C, 2Hr
Date : Anonymous submitted on 26-Aug-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : 3T3-L1 cells
Amount used : 120μg
Blocking : 5% skim milk, 25°C, 2Hr
Primary Antibody : 1:1000, 25°C, 2Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169