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Adiponectin antibody

Orthogonal
Anti-Adiponectin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23455
Anti-Adiponectin antibody used in Western Blot (WB). GTX23455
Anti-Adiponectin antibody used in Western Blot (WB). GTX23455
Anti-Adiponectin antibody used in Western Blot (WB). GTX23455

Cat. No. GTX23455

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, IHC

Reactivity

 Human, Mouse
Package
200 μg ($399)
See all Adiponectin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/ml
ICC/IF 2 μg/ml
IHC-P Assay dependent
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptides corresponding to the residues E(18) D D V T T T E E L A P A L V(32) and F(187) T Y D Q Y Q E K N V D Q A(200) of mouse Adiponectin.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_367259

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adiponectin, C1Q and collagen domain containing , 30kDa , APN , Acdc , Acrp30 , Ad , Adid , GBP28 , adipo , apM1

Cellular Localization

Secreted

Background

Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin and adiponectin protein of 30 kDa. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, mRNA levels of Adiponectin have been shown to be induced over 100-fold. Studies indicate that Adiponectin enhances the ability of sub-physiological levels of insulin to suppress glucose production, thus linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol-3-kinase (PI3-K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. mRNA levels of Adiponectin have been shown to be significantly reduced in adipose tissue of obese patients with Type 2 diabetes relative to control subjects. Structural similarities to TNFalpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Because Adiponectin is down-regulated in various forms of obesity and its structural similarity to TNFalpha, it is currently being investigated as an important regulator of whole body energy homeostasis.

Database

Research Area

DATA IMAGES

Anti-Adiponectin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23455

GTX23455 ICC/IF Image

Orthogonal

ICC/IF analysis of 3T3-L1 cells differentiated with Adipogenesis Supplement using GTX23455 Adiponectin antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Anti-Adiponectin antibody used in Western Blot (WB). GTX23455

GTX23455 WB Image

WB analysis of mouse serum using GTX23455 Adiponectin antibody.

Anti-Adiponectin antibody used in Western Blot (WB). GTX23455

GTX23455 WB Image

WB analysis of tissue extract (30 ug lysate) of mouse adipose using GTX23455 Adiponectin antibody.
Dilution : 2 μg/ml

Anti-Adiponectin antibody used in Western Blot (WB). GTX23455

GTX23455 WB Image

The data was published in the journal Physiol Rep in 2018.PMID: 29446245

REFERENCE

Application Reference

Tang YH et al. Int J Mol Sci 2022; 23 (21) Genetic and Functional Effects of Adiponectin in Type 2 Diabetes Mellitus Development.
Application : WB, IHC-P
Reactivity : Mouse
Martinez-Huenchullan SF et al. Physiol Rep 2018; 6 (20) : e13848 Skeletal muscle adiponectin induction depends on diet, muscle type/activity, and exercise modality in C57BL/6 mice.
Application : WB
Reactivity : Mouse
Martinez-Huenchullan SF et al. Physiol Rep 2018; 6 (4) Differential metabolic effects of constant moderate versus high intensity interval training in high-fat fed mice: possible role of muscle adiponectin.
Application : WB
Reactivity : Mouse
Harada N et al. Gut Microbes 2016; 7 (6) : 533-539 Hypogonadism alters cecal and fecal microbiota in male mice.
Application : WB
Reactivity : Mouse
Harada N et al. Gut Microbes 2016; (Epub) Hypogonadism alters cecal and fecal microbiota in male mice.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399