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Aggrecan antibody [GT1267]

Recombinant antibody
Anti-Aggrecan antibody [GT1267] used in Western Blot (WB). GTX03179
Anti-Aggrecan antibody [GT1267] used in IHC (Paraffin sections) (IHC-P). GTX03179
Anti-Aggrecan antibody [GT1267] used in IHC (Paraffin sections) (IHC-P). GTX03179
Anti-Aggrecan antibody [GT1267] used in Western Blot (WB). GTX03179

Cat. No. GTX03179

Reference 2

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1267

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($439)
See all Aggrecan products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

261 kDa. ( Note )

Observed MW

~400 kDa. The observed M.W. is based on the following publication. PMID: 31972963

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Aggrecan.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aggrecan , AGC1 , AGCAN , CSPG1 , CSPGCP , MSK16 , SEDK , SSOAOD

Background

This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Aggrecan antibody [GT1267] used in Western Blot (WB). GTX03179

GTX03179 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Aggrecan antibody [GT1267] (GTX03179) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
The observed M.W. is based on the publication: PMID: 31972963

Anti-Aggrecan antibody [GT1267] used in IHC (Paraffin sections) (IHC-P). GTX03179

GTX03179 IHC-P Image

IHC-P analysis of rat bone marrow tissue section using GTX03179 Aggrecan antibody [GT1267].
Dilution : 1:100

Anti-Aggrecan antibody [GT1267] used in IHC (Paraffin sections) (IHC-P). GTX03179

GTX03179 IHC-P Image

IHC-P analysis of mouse bone marrow tissue section using GTX03179 Aggrecan antibody [GT1267].
Dilution : 1:100

Anti-Aggrecan antibody [GT1267] used in Western Blot (WB). GTX03179

GTX03179 WB Image

WB analysis of various samples using GTX03179 Aggrecan antibody [GT1267].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

N Meng et al. J Orthop Surg Res 2023; PLXNC1 interference alleviates the inflammatory injury, apoptosis and extracellular matrix degradation of IL-1β-exposed chondrocytes via suppressing GRP78 expression
Application : WB
Chang YH et al. Stem Cells Int 2021; 2021 : 2989054 Human Umbilical Mesenchymal Stromal Cells Mixed with Hyaluronan Transplantation Decreased Cartilage Destruction in a Rabbit Osteoarthritis Model.
Application : IHC-P
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REVIEW

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Package List Price ($)
$ 439