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Aggrecan antibody

Anti-Aggrecan antibody used in Western Blot (WB). GTX54920
Anti-Aggrecan antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54920
Anti-Aggrecan antibody used in IHC (Paraffin sections) (IHC-P). GTX54920

Cat. No. GTX54920

Reference 12

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Pig
Package
100 μl ($399)
See all Aggrecan products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF Assay dependent
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

261 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of Aggrecan. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887906

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aggrecan , AGC1 , AGCAN , CSPG1 , CSPGCP , MSK16 , SEDK , SSOAOD

Background

This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Aggrecan antibody used in Western Blot (WB). GTX54920

GTX54920 WB Image

WB analysis of various tissue lysates using GTX54920 Aggrecan antibody.
(A): CT26
(B): C6
(C): Hela

Anti-Aggrecan antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54920

GTX54920 ICC/IF Image

ICC/IF analysis of C6 cells using GTX54920 Aggrecan antibody.
Red: Phalloidin
Blue: DAPI

Anti-Aggrecan antibody used in IHC (Paraffin sections) (IHC-P). GTX54920

GTX54920 IHC-P Image

IHC-P analysis of formalin fixed human lung cancer tissue section using GTX54920 Aggrecan antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

Application Reference

Sun Q et al. Bone Rep 2024; 20 : 101744 Strontium ranelate retards disc degradation and improves endplate and bone micro-architecture in ovariectomized rats with lumbar fusion induced - Adjacent segment disc degeneration.
Ping SH et al. Iran J Basic Med Sci 2022; 25 (10) : 1228-1233 Protective effects of equol on the cartilage and subchondral bone in ovariectomized rats with osteoarthritis.
Csukasi F et al. Front Cell Dev Biol 2022; 10 : 963389 Skeletal diseases caused by mutations in PTH1R show aberrant differentiation of skeletal progenitors due to dysregulation of DEPTOR.
Application : IHC-P
Reactivity : Human
Qi Sun et al. Arthritis Res Ther 2021; 23 (1) : 152 Denosumab alleviates intervertebral disc degeneration adjacent to lumbar fusion by inhibiting endplate osteochondral remodeling and vertebral osteoporosis in ovariectomized rats.
Application : IHC-P
Ping SH et al. Bosn J Basic Med Sci 2021; 21 (3) : 284-293 Raloxifene inhibits the overexpression of TGF-β1 in cartilage and regulates the metabolism of subchondral bone in rats with osteoporotic osteoarthritis.
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REVIEW

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Application Reference
Sun Q et al. Bone Rep 2024; 20:101744 Strontium ranelate retards disc degradation and improves endplate and bone micro-architecture in ovariectomized rats with lumbar fusion induced - Adjacent segment disc degeneration.
Ping SH et al. Iran J Basic Med Sci 2022; 25 (10):1228-1233 Protective effects of equol on the cartilage and subchondral bone in ovariectomized rats with osteoarthritis.
Csukasi F et al. Front Cell Dev Biol 2022; 10:963389 Skeletal diseases caused by mutations in PTH1R show aberrant differentiation of skeletal progenitors due to dysregulation of DEPTOR.
Application : IHC-P
Reactivity : Human
Qi Sun et al. Arthritis Res Ther 2021; 23 (1):152 Denosumab alleviates intervertebral disc degeneration adjacent to lumbar fusion by inhibiting endplate osteochondral remodeling and vertebral osteoporosis in ovariectomized rats.
Application : IHC-P
Ping SH et al. Bosn J Basic Med Sci 2021; 21 (3):284-293 Raloxifene inhibits the overexpression of TGF-β1 in cartilage and regulates the metabolism of subchondral bone in rats with osteoporotic osteoarthritis.
Lee SS et al. Int J Med Sci 2021; 18 (10):2217-2227 A novel 3D histotypic cartilage construct engineered by supercritical carbon dioxide decellularized porcine nasal cartilage graft and chondrocytes exhibited chondrogenic capability in vitro.
Chen CY et al. Biomolecules 2021; 11 (1) Kartogenin Enhances Chondrogenic Differentiation of MSCs in 3D Tri-Copolymer Scaffolds and the Self-Designed Bioreactor System.
Application : IHC-P
Reactivity : Rat
Chen YT et al. J Tissue Eng Regen Med 2021; 15 (2):163-175 3D composite engineered using supercritical CO2 decellularized porcine cartilage scaffold, chondrocytes, and PRP: Role in articular cartilage regeneration.
Application : IHC-P
Reactivity : Pig
Zhang L et al. Stem Cell Res Ther 2020; 11 (1):269 Impaired autophagy triggered by HDAC9 in mesenchymal stem cells accelerates bone mass loss.
Shao LT et al. Bone Joint Res 2020; 9 (10):675-688 Parathyroid hormone (1-34) ameliorates cartilage degeneration and subchondral bone deterioration in collagenase-induced osteoarthritis model in mice.
Application : IHC-P
Reactivity : Mouse
Muhammad SA et al. Cartilage 2019;:1947603519876333 Optimization of Protocol for Isolation of Chondrocytes from Human Articular Cartilage.
Application : ICC/IF
Chang YH et al. Tzu Chi Medical Journal 2018; (Epub) Human umbilical cord-derived mesenchymal stem cells reduce monosodium iodoacetate-induced apoptosis in cartilage.
Application : IHC-P
Reactivity : Human
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399