GeneTex
United States (US)

Agrin antibody [Agr-131]

Cat No. GTX12362

Host Mouse
Clonality Monoclonal
Clone Name Agr-131
Isotype IgG1
Application WB, ICC/IF, IHC-Fr, RIA
Reactivity Mouse, Rat
APPLICATION

Application Note

ICC: Use at a dilution of 1/50 - 1/1000. IHC-Fr: Use at an assay dependent dilution. RIA: Use at a concentration of 1 μg/ml. WB: Use at a concentration of 2 μg/ml. Predicted molecular weight: 208.6 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

209 kDa. ( Note )

Specificity/Sensitivity

GTX12362 detects all isoforms of agrin. This antibody recognizes all N1 isoforms but, consistent with the epitope mapping investigations, fails to see the N4 protein. Furthermore, the antibody will detect the X, Y and Z splice variants.
PROPERTIES

Form

Liquid

Buffer

Preservative: NoneConstituents: 50% Glycerol, PBS, 0.1mM PMSF. pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Recombinant rat agrin, C-terminal construct.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Agrin,Agr,Agrn

Cellular Localization

Synaptic basal lamina at the neuromuscular junction.

Background

Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.

Database

Research Area

Package List Price ($)
$ 329