Application Note
ICC: Use at a dilution of 1/50 - 1/1000. IHC-Fr: Use at an assay dependent dilution. RIA: Use at a concentration of 1 μg/ml. WB: Use at a concentration of 2 μg/ml. Predicted molecular weight: 208.6 kDa. Optimal dilutions/concentrations should be determined by the end user.
Calculated MW
Product Note
GTX12362 detects all isoforms of agrin. This antibody recognizes all N1 isoforms but, consistent with the epitope mapping investigations, fails to see the N4 protein. Furthermore, the antibody will detect the X, Y and Z splice variants.
Form
Liquid
Buffer
50% Glycerol, PBS pH7.2, 0.1mM PMSF
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Rat
Immunogen
Recombinant rat agrin, C-terminal construct.
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
agrin , AGR
Cellular Localization
Cell junction, synapse , Cell membrane
Background
may play a role in synapse development and regeneration [RGD, Feb 2006]
Database
Research Area