GeneTex
United States (US)

Agrin antibody [Agr-33]

Agrin-antibody-Agr-33_IHC_GTX12358-1_18121409_657.jpg

Cat No. GTX12358

Host Mouse
Clonality Monoclonal
Clone Name Agr-33
Isotype IgG1
Application WB, ICC/IF, IHC
Reactivity Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

209 kDa. ( Note )

Specificity/Sensitivity

GTX12358 detects all isoforms of agrin.
PROPERTIES

Form

Liquid

Buffer

Preservative: NoneConstituents: 50% Glycerol, PBS, 0.1mM PMSF. pH 7.2

Concentration

1mg/ml

Antigen Species

Rat

Immunogen

Recombinant rat agrin, C-terminal construct.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Agrn, AGR, Agrin, 25592

Cellular Localization

Synaptic basal lamina at the neuromuscular junction.

Background

Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.
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Database

Research Area

DATA IMAGES
Agrin-antibody-Agr-33_IHC_GTX12358-1_18121409_657.jpg

GTX12358 IHC Image

Immunohistochemistry analysis of rat skeletal muscle probed with Agrin, mAb at 1:100.