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Ajuba antibody

Anti-Ajuba antibody used in Western Blot (WB). GTX48743
Anti-Ajuba antibody used in Western Blot (WB). GTX48743

Cat No. GTX48743

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, ELISA

Reactivity

Human
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2500
IP 1:100
ELISA 1:20000 - 1:80000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 224-239 of Human Ajuba.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ajuba LIM protein , JUB

Cellular Localization

Cytoplasm, cytoskeleton,Cell membrane,Cell junction,Nucleus

Background

Human Ajuba (also called JUB protein and ajuba homolog isoform 1) is a LIM domain protein suggested to bind and regulate the activity of Aurora A. Aurora A, which is involved in cell cycle regulation, is upregulated during mitosis, localizing to the centrosomes and microtubule regions proximal to the centrosomes.

Database

Research Area

DATA IMAGES

Anti-Ajuba antibody used in Western Blot (WB). GTX48743

GTX48743 WB Image

Western blot using Affinity Purified anti-Ajuba antibody shows detection of Ajuba-RFP fusion protein in cell lysates (arrow-head). Lanes correspond to 1) vector only trans-fection, 2) human Ajuba-RFP, 3) mouse Ajuba-RFP, and 4) mock transfection. Approximately 50 μg of each lysate was loaded per lane for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1:1,700 dilution of anti-Ajuba antibody.

Anti-Ajuba antibody used in Western Blot (WB). GTX48743

GTX48743 WB Image

Western blot using Affinity Purified anti-Ajuba antibody shows detection of a 57-kDa band consistent with the expected MW for Ajuba (arrowhead). Lanes correspond to 1) HeLa nuclear extract, and 2) HeLa, 3) A431, 4) Jurkat and 5) 293 whole cell lysates. Immunoprecipitation of Ajuba followed by western blotting may result in cleaner background staining. Approximately 5 μg of each preparation was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-Ajuba antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 60-sec exposure time.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf