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Aladin antibody [N2C2], Internal

Anti-Aladin antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100475
Anti-Aladin antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100475
Anti-Aladin antibody [N2C2], Internal used in Western Blot (WB). GTX100475

Cat No. GTX100475

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

293T , A431

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Aladin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

aladin WD repeat nucleoporin,AAA,AAASb,ADRACALA,ADRACALIN,ALADIN,GL003

Background

The protein encoded by this gene is a member of the WD-repeat family of regulatory proteins and may be involved in normal development of the peripheral and central nervous system. The encoded protein is part of the nuclear pore complex and is anchored there by NDC1. Defects in this gene are a cause of achalasia-addisonianism-alacrima syndrome (AAAS), also called triple-A syndrome or Allgrove syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
Anti-Aladin antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100475

GTX100475 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Aladin(GTX100475) antibody at 1:500 dilution.

Anti-Aladin antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100475

GTX100475 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Aladin(GTX100475) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Aladin antibody [N2C2], Internal used in Western Blot (WB). GTX100475

GTX100475 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
7.5% SDS PAGE
GTX100475 diluted at 1:1000

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169