Albumin antibody

Name: Albumin antibody
Brand: GeneTex
SKU: GTX102419
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Albumin antibody used in Western Blot (WB). GTX102419

Anti-Albumin antibody used in IHC (Paraffin sections) (IHC-P). GTX102419

Anti-Albumin antibody used in IHC (Frozen sections) (IHC-Fr). GTX102419

Anti-Albumin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102419

Cat. No. GTX102419

Reference 18

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, IHC
Reactivity	Human, Mouse, Rat, Cat, Dog
Package	100 μl ($399), 25 μl ($169)

See all Albumin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:100000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000
IHC	Assay dependent

Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Predict Reactivity

Rabbit, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Albumin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949626

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

albumin , HSA , PRO0883 , PRO0903 , PRO1341

Cellular Localization

Secreted

Background

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq]

Database

Gene ID: 213 ALB
UniProt: P02768 ALB

Research Area

DATA IMAGES

GTX102419 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (GTX102419) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX102419 IHC-P Image

Albumin antibody detects Albumin protein at cytoplasm in human hepatocellular carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
Albumin antibody (GTX102419) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102419 IHC-Fr Image

Albumin antibody detects Albumin protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Frozen section of rat liver.
Albumin antibody (GTX102419) diluted at 1:200.

GTX102419 IHC-P Image

Albumin antibody detects secreted Albumin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Albumin stained by Albumin antibody (GTX102419) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102419 WB Image

Human plasma (1 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (GTX102419) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102419 IHC-P Image

Albumin antibody detects secreted Albumin protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Albumin stained by Albumin antibody (GTX102419) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102419 ICC/IF Image

Albumin antibody detects Albumin protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Albumin protein stained by Albumin antibody (GTX102419) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX102419 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (GTX102419) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX102419 IHC-P Image

Albumin antibody detects Albumin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog liver.
Albumin stained by Albumin antibody (GTX102419) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102419 IHC-P Image

Albumin antibody detects secreted Albumin protein by immunohistochemical analysis.
Sample: Paraffin-embedded dog kidney.
Albumin stained by Albumin antibody (GTX102419) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102419 WB Image

PG-4 whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (GTX102419) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102419 ICC/IF Image

Albumin antibody detects Albumin protein at cytoplasm by immunofluorescent analysis.
Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Albumin stained by Albumin antibody (GTX102419) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX102419 ICC/IF Image

Albumin antibody detects Albumin protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Albumin stained by Albumin antibody (GTX102419) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX102419 IHC-P Image

Albumin antibody detects Albumin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat liver.
Albumin stained by Albumin antibody (GTX102419) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102419 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (GTX102419) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.