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Albumin antibody

Orthogonal
Anti-Albumin antibody used in Western Blot (WB). GTX102419
Anti-Albumin antibody used in Western Blot (WB). GTX102419
Anti-Albumin antibody used in IHC (Paraffin sections) (IHC-P). GTX102419
Anti-Albumin antibody used in IHC (Frozen sections) (IHC-Fr). GTX102419
Anti-Albumin antibody used in IHC (Paraffin sections) (IHC-P). GTX102419
Anti-Albumin antibody used in Western Blot (WB). GTX102419
Anti-Albumin antibody used in IHC (Paraffin sections) (IHC-P). GTX102419
Anti-Albumin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102419
Anti-Albumin antibody used in Western Blot (WB). GTX102419
Anti-Albumin antibody used in IHC (Paraffin sections) (IHC-P). GTX102419
Anti-Albumin antibody used in IHC (Paraffin sections) (IHC-P). GTX102419
Anti-Albumin antibody used in Western Blot (WB). GTX102419
Anti-Albumin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102419
Anti-Albumin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102419
Anti-Albumin antibody used in IHC (Paraffin sections) (IHC-P). GTX102419

Cat. No. GTX102419

Reference 19
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC, in vitro

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all Albumin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:100000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC Assay dependent
in vitro Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Predict Reactivity

Rabbit, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Albumin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949626

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

albumin , HSA , PRO0883 , PRO0903 , PRO1341

Cellular Localization

Secreted

Background

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Albumin antibody used in Western Blot (WB). GTX102419

GTX102419 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (GTX102419) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Albumin antibody used in Western Blot (WB). GTX102419

GTX102419 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (GTX102419) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Albumin antibody used in IHC (Paraffin sections) (IHC-P). GTX102419

GTX102419 IHC-P Image

Albumin antibody detects Albumin protein at cytoplasm in human hepatocellular carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
Albumin antibody (GTX102419) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Albumin antibody used in IHC (Frozen sections) (IHC-Fr). GTX102419

GTX102419 IHC-Fr Image

Albumin antibody detects Albumin protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Frozen section of rat liver.
Albumin antibody (GTX102419) diluted at 1:200.
Anti-Albumin antibody used in IHC (Paraffin sections) (IHC-P). GTX102419

GTX102419 IHC-P Image

Albumin antibody detects secreted Albumin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Albumin stained by Albumin antibody (GTX102419) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Albumin antibody used in Western Blot (WB). GTX102419

GTX102419 WB Image

Human plasma (1 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (GTX102419) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Albumin antibody used in IHC (Paraffin sections) (IHC-P). GTX102419

GTX102419 IHC-P Image

Albumin antibody detects secreted Albumin protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Albumin stained by Albumin antibody (GTX102419) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Albumin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102419

GTX102419 ICC/IF Image

Albumin antibody detects Albumin protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Albumin protein stained by Albumin antibody (GTX102419) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Albumin antibody used in Western Blot (WB). GTX102419

GTX102419 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (GTX102419) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Albumin antibody used in IHC (Paraffin sections) (IHC-P). GTX102419

GTX102419 IHC-P Image

Albumin antibody detects Albumin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog liver.
Albumin stained by Albumin antibody (GTX102419) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Albumin antibody used in IHC (Paraffin sections) (IHC-P). GTX102419

GTX102419 IHC-P Image

Albumin antibody detects secreted Albumin protein by immunohistochemical analysis.
Sample: Paraffin-embedded dog kidney.
Albumin stained by Albumin antibody (GTX102419) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Albumin antibody used in Western Blot (WB). GTX102419

GTX102419 WB Image

PG-4 whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (GTX102419) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Albumin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102419

GTX102419 ICC/IF Image

Albumin antibody detects Albumin protein at cytoplasm by immunofluorescent analysis.
Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Albumin stained by Albumin antibody (GTX102419) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Albumin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102419

GTX102419 ICC/IF Image

Albumin antibody detects Albumin protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Albumin stained by Albumin antibody (GTX102419) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Albumin antibody used in IHC (Paraffin sections) (IHC-P). GTX102419

GTX102419 IHC-P Image

Albumin antibody detects Albumin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat liver.
Albumin stained by Albumin antibody (GTX102419) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

MC Wu et al. PLoS One 2023; TAT peptide at treatment-level concentrations crossed brain endothelial cell monolayer independent of receptor-mediated endocytosis or peptide-inflicted barrier disruption
Application : in vitro
T Chida et al. JCI Insight 2023; Persistent hepatic IFN system activation in HBV-HDV infection determines viral replication dynamics and therapeutic response
Application : WB
Reactivity : Human
Fujino M et al. JCI Insight 2023; 8 (6) Transcription factor c-Maf deletion improves streptozotocin-induced diabetic nephropathy by directly regulating Sglt2 and Glut2.
Application : IHC-P
Reactivity : Mouse
Yuan Y et al. Nat Commun 2022; 13 (1) : 5237 PIM1 promotes hepatic conversion by suppressing reprogramming-induced ferroptosis and cell cycle arrest.
Lai H et al. Cancers (Basel) 2022; 14 (15) Protein Panel of Serum-Derived Small Extracellular Vesicles for the Screening and Diagnosis of Epithelial Ovarian Cancer.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

Albumin antibody Cat. No. GTX102419

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Albumin antibody used in Western Blot (WB). GTX102419
Date : Anonymous submitted on 18-Jun-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 15 μg
Blocking : 1% skim milk in TBS, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Lane Description : Lane 1: HepG2 spheroid; Lane 2: HepG2 monolayer
Submit a Review
Application Reference
MC Wu et al. PLoS One 2023; TAT peptide at treatment-level concentrations crossed brain endothelial cell monolayer independent of receptor-mediated endocytosis or peptide-inflicted barrier disruption
Application : in vitro
T Chida et al. JCI Insight 2023; Persistent hepatic IFN system activation in HBV-HDV infection determines viral replication dynamics and therapeutic response
Application : WB
Reactivity : Human
Fujino M et al. JCI Insight 2023; 8 (6) Transcription factor c-Maf deletion improves streptozotocin-induced diabetic nephropathy by directly regulating Sglt2 and Glut2.
Application : IHC-P
Reactivity : Mouse
Yuan Y et al. Nat Commun 2022; 13 (1):5237 PIM1 promotes hepatic conversion by suppressing reprogramming-induced ferroptosis and cell cycle arrest.
Lai H et al. Cancers (Basel) 2022; 14 (15) Protein Panel of Serum-Derived Small Extracellular Vesicles for the Screening and Diagnosis of Epithelial Ovarian Cancer.
Application : WB
Reactivity : Human
Tu YF et al. Cell Death Discov 2021; 7 (1):150 Endothelial-specific insulin receptor substrate-1 overexpression worsens neonatal hypoxic-ischemic brain injury via mTOR-mediated tight junction disassembly.
Application : IHC-Fr
Kang HK et al. Cells 2021; 10 (5) Establishing a 3D In Vitro Hepatic Model Mimicking Physiologically Relevant to In Vivo State.
Application : ICC/IF
Reactivity : Human
Wu MC et al. Neuroreport 2021; 32 (11):957-964 Evans blue dye as an indicator of albumin permeability across a brain endothelial cell monolayer in vitro.
Wang YL et al. Environ Health Perspect 2021; 129 (5):57003 The Kidney-Related Effects of Polystyrene Microplastics on Human Kidney Proximal Tubular Epithelial Cells HK-2 and Male C57BL/6 Mice.
Application : WB
Reactivity : Human
Matt C Biery et al. J Exp Neurol 2020; 1 (4):158-167 A Protocol for the Generation of Treatment-na?ve Biopsy-derived Diffuse Intrinsic Pontine Glioma and Diffuse Midline Glioma Models.
Application : IHC-P
Reactivity : Mouse
Gerardo A Lopez-Mu?oz et al. Nanomaterials (Basel) 2020; 10 (12) Direct and Label-Free Monitoring of Albumin in 2D Fatty Liver Disease Model Using Plasmonic Nanogratings.
Application : ICC/IF
Reactivity : Mouse
Gan P et al. Sci Rep 2020; 10 (1):7605 Allelic variants between mouse substrains BALB/cJ and BALB/cByJ influence mononuclear cardiomyocyte composition and cardiomyocyte nuclear ploidy.
Reactivity : Mouse
Mohamed Elbadawy et al. Biomaterials 2020; (Epub) Efficacy of primary liver organoid culture from different stages of non-alcoholic steatohepatitis (NASH) mouse model.
Application : ICC/IF
Reactivity : Mouse
Mauffrey P et al. Nature 2019; 569 (7758):672-678 Progenitors from the central nervous system drive neurogenesis in cancer.
Application : ICC/IF
Reactivity : Mouse
AshleighWillis et al. Journal of Neuroscience Methods 2018; (Epub) Distortion of protein analysis in primary neuronal cultures by serum albumin from culture medium: a methodological approach to improve target protein quantification.
Application : WB
Reactivity : Mouse
Chung YH et al. Lab Invest 2018; (Epub) BMP-2 restoration aids in recovery from liver fibrosis by attenuating TGF-β1 signaling.
Application : ICC/IF
Reactivity : Human
Lee YC et al. Mol Neurobiol 2018; (Epub) Hypoxia-Preconditioned Human Umbilical Vein Endothelial Cells Protect Against Neurovascular Damage After Hypoxic Ischemia in Neonatal Brain.
Application : IHC
Reactivity : Rat
Shih SC et al. Am J Transl Res 2017; 9 (3):1114-1126 Pigment epithelium-derived factor (PEDF) peptide promotes the expansion of hepatic stem/progenitor cells via ERK and STAT3-dependent signaling.
Kurihara C et al. Biotechnol Rep 2016; 12:26-32 An easy method for preparation of Cre-loxP regulated fluorescent adenoviral expression vectors and its application for direct reprogramming into hepatocytes.
Application : ICC/IF
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169