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Albumin antibody

Anti-Albumin antibody used in Western Blot (WB). GTX102419
Anti-Albumin antibody used in IHC (Frozen sections) (IHC-Fr). GTX102419
Anti-Albumin antibody used in Western Blot (WB). GTX102419
Anti-Albumin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102419
Anti-Albumin antibody used in Western Blot (WB). GTX102419
Anti-Albumin antibody used in IHC (Paraffin sections) (IHC-P). GTX102419
Anti-Albumin antibody used in Western Blot (WB). GTX102419

Cat No. GTX102419

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IHC
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:100000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

human plasma , HepG2 , mouse Liver

Predict Reactivity

Rabbit, Cat, Dog, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Albumin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

albumin , HSA , PRO0883 , PRO0903 , PRO1341

Cellular Localization

Secreted

Background

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Albumin antibody used in Western Blot (WB). GTX102419

GTX102419 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (GTX102419) diluted at 1:1000.

Anti-Albumin antibody used in IHC (Frozen sections) (IHC-Fr). GTX102419

GTX102419 IHC-Fr Image

Albumin antibody detects Albumin protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Frozen section of rat liver.
Albumin antibody (GTX102419) diluted at 1:200.

Anti-Albumin antibody used in Western Blot (WB). GTX102419

GTX102419 WB Image

Human plasma (1 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (GTX102419) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Albumin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102419

GTX102419 ICC/IF Image

Albumin antibody detects Albumin protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Albumin protein stained by Albumin antibody (GTX102419) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Albumin antibody used in Western Blot (WB). GTX102419

GTX102419 WB Image

Sample (20 ug of whole cell lysate)
A: mouse Liver
7.5% SDS PAGE
GTX102419 diluted at 1:10000

Anti-Albumin antibody used in IHC (Paraffin sections) (IHC-P). GTX102419

GTX102419 IHC-P Image

Albumin antibody detects Albumin protein at cytoplasm in human hepatocellular carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
Albumin antibody (GTX102419) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Albumin antibody used in Western Blot (WB). GTX102419

GTX102419 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (GTX102419) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE
REVIEW

Albumin antibody Cat No. GTX102419

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Jun-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 15 μg
Blocking : 1% skim milk in TBS, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Lane Description : Lane 1: HepG2 spheroid; Lane 2: HepG2 monolayer
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169