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Aldolase A antibody [AT3F9]

Anti-Aldolase A antibody [AT3F9] used in Flow cytometry (FACS). GTX16497
Anti-Aldolase A antibody [AT3F9] used in Western Blot (WB). GTX16497
Anti-Aldolase A antibody [AT3F9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16497

Cat No. GTX16497

Host

Mouse

Clonality

Monoclonal

Clone Name

AT3F9

Isotype

IgG2a

Application

WB, ICC/IF, FACS, ELISA

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

WB: 1:1000. ICC/IF: 1:200. FACS: 1:200

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH7.4), 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human ALDOA (1-364aa)

Purification

By protein-A affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

aldolase, fructose-bisphosphate A , ALDA , GSD12 , HEL-S-87p

Background

This gene encodes a member of the class I fructose-bisphosphate aldolase protein family. The encoded protein is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Mutations in this gene have been associated with Glycogen Storage Disease XII, an autosomal recessive disorder associated with hemolytic anemia. Disruption of this gene also plays a role in the progression of multiple types of cancers. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Sep 2017]

Database

Research Area

DATA IMAGES

Anti-Aldolase A antibody [AT3F9] used in Flow cytometry (FACS). GTX16497

GTX16497 FACS Image

FACS analysis of A549 cells using an isotype control (black) or Aldolase A antibody [AT3F9] at 2-5ug for 1,000,000 cells (red).

Anti-Aldolase A antibody [AT3F9] used in Western Blot (WB). GTX16497

GTX16497 WB Image

WB analysis of (1) recombinant human Aldolase A, (2) HeLa and (3) HepG2 lysates (40ug of lysates) using Aldolase A antibody [AT3F9] at a dilution of 1:1000.

Anti-Aldolase A antibody [AT3F9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16497

GTX16497 ICC/IF Image

ICC/IF analysis of A549 cells using DAPI (blue) and Aldolase A antibody [AT3F9] at a dilution of 1:200 (green).

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339