GeneTex
United States (US)

Aldolase A antibody [AT3F9]

GTX16497_FACS_18121410_494.jpg
GTX16497_WB_18121410_586.jpg
GTX16497_ICCIF_18121410_544.jpg

Cat No. GTX16497

Host Mouse
Clonality Monoclonal
Clone Name AT3F9
Isotype IgG2a
Application WB, ICC/IF, FACS, ELISA
Reactivity Human
APPLICATION

Application Note

WB: 1:1000. ICC/IF: 1:200. FACS: 1:200

Calculated MW

39 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human ALDOA (1-364aa)

Purification

By protein-A affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Aldolase, Fructose-Bisphosphate A,Alda,Gsd12,Hel-S-87P,Aldoa

Background

The protein encoded by this gene, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES
GTX16497_FACS_18121410_494.jpg

GTX16497 FACS Image

FACS analysis of A549 cells using an isotype control (black) or Aldolase A antibody [AT3F9] at 2-5ug for 1,000,000 cells (red).

GTX16497_WB_18121410_586.jpg

GTX16497 WB Image

WB analysis of (1) recombinant human Aldolase A, (2) HeLa and (3) HepG2 lysates (40ug of lysates) using Aldolase A antibody [AT3F9] at a dilution of 1:1000.

GTX16497_ICCIF_18121410_544.jpg

GTX16497 ICC/IF Image

ICC/IF analysis of A549 cells using DAPI (blue) and Aldolase A antibody [AT3F9] at a dilution of 1:200 (green).