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Aldolase A antibody

Anti-Aldolase A antibody used in IHC (Paraffin sections) (IHC-P). GTX101408
Anti-Aldolase A antibody used in IHC (Paraffin sections) (IHC-P). GTX101408
Anti-Aldolase A antibody used in Western Blot (WB). GTX101408
Anti-Aldolase A antibody used in Western Blot (WB). GTX101408

Cat. No. GTX101408

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Aldolase A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain , rat brain

Predict Reactivity

Rabbit, Bovine, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Aldolase A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885229

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldolase, fructose-bisphosphate A , ALDA , GSD12 , HEL-S-87p

Background

This gene encodes a member of the class I fructose-bisphosphate aldolase protein family. The encoded protein is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Mutations in this gene have been associated with Glycogen Storage Disease XII, an autosomal recessive disorder associated with hemolytic anemia. Disruption of this gene also plays a role in the progression of multiple types of cancers. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Sep 2017]

Database

Research Area

DATA IMAGES

Anti-Aldolase A antibody used in IHC (Paraffin sections) (IHC-P). GTX101408

GTX101408 IHC-P Image

Aldolase A antibody detects Aldolase A protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Aldolase A antibody (GTX101408) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Aldolase A antibody used in IHC (Paraffin sections) (IHC-P). GTX101408

GTX101408 IHC-P Image

Aldolase A antibody detects Aldolase A protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Aldolase A antibody (GTX101408) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Aldolase A antibody used in Western Blot (WB). GTX101408

GTX101408 WB Image

Aldolase A antibody detects Aldolase A protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
Aldolase A antibody (GTX101408) dilution: 1:1000

Anti-Aldolase A antibody used in Western Blot (WB). GTX101408

GTX101408 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase A antibody (GTX101408) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Ruis K et al. J Biol Chem 2022; 298 (7) : 101992 Delineation of a minimal topoisomerase II binding protein?1?for regulated activation of ATR at DNA double-strand breaks.
Application : WB
Reactivity : Human
Elisa De Tomi et al. Int J Mol Sci 2022; 23 (3) Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
Application : WB
Reactivity : Human
Kim A et al. DNA Repair (Amst) 2020; 96 : 102973 Biochemical analysis of TOPBP1 oligomerization.
Chang SJ et al. Arch Biochem Biophys 2018; 647 : 10-32 Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma.
Application : WB
Reactivity : Human
Cardouat G et al. Biochim Biophys Acta 2017; (Epub) Ectopic adenine nucleotide translocase activity controls extracellular ADP levels and regulates the F1-ATPase-mediated HDL endocytosis pathway on hepatocytes.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169