APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , mouse brain , rat brain
Predict Reactivity
Rabbit, Bovine, Japanese Medaka, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.58 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Aldolase A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885229
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
aldolase, fructose-bisphosphate A , ALDA , GSD12 , HEL-S-87p
Background
This gene encodes a member of the class I fructose-bisphosphate aldolase protein family. The encoded protein is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Mutations in this gene have been associated with Glycogen Storage Disease XII, an autosomal recessive disorder associated with hemolytic anemia. Disruption of this gene also plays a role in the progression of multiple types of cancers. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Sep 2017]
Database
Research Area
DATA IMAGES
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GTX101408 IHC-P Image
Aldolase A antibody detects Aldolase A protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Aldolase A antibody (GTX101408) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101408 WB Image
Aldolase A antibody detects Aldolase A protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
Aldolase A antibody (GTX101408) dilution: 1:1000
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GTX101408 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase A antibody (GTX101408) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101408 IHC-P Image
Aldolase A antibody detects Aldolase A protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Aldolase A antibody (GTX101408) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
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