GeneTex
United States (US)

Aldolase A antibody

GTX101408_41031_IHC_R_19011506_820.jpg
GTX101408_41031_WB_19011506_684.jpg
GTX101408_41031_20170713_WB_M_R_19011506_241.jpg
GTX101408_41031_IHC_M_19011506_601.jpg

Cat No. GTX101408

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain , rat brain

Predict Reactivity

Rabbit, Bovine, Japanese Medaka, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Aldolase A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ALDOA Antibody, aldolase A, fructose-bisphosphate Antibody, ALDA Antibody, GSD12 Antibody

Background

This gene encodes a member of the class I fructose-bisphosphate aldolase protein family. The encoded protein is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Mutations in this gene have been associated with Glycogen Storage Disease XII, an autosomal recessive disorder associated with hemolytic anemia. Disruption of this gene also plays a role in the progression of multiple types of cancers. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Sep 2017]

Database

Research Area

DATA IMAGES
GTX101408_41031_IHC_R_19011506_820.jpg

GTX101408 IHC-P Image

Aldolase A antibody detects Aldolase A protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Aldolase A antibody (GTX101408) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101408_41031_WB_19011506_684.jpg

GTX101408 WB Image

Aldolase A antibody detects Aldolase A protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
Aldolase A antibody (GTX101408) dilution: 1:1000

GTX101408_41031_20170713_WB_M_R_19011506_241.jpg

GTX101408 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase A antibody (GTX101408) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101408_41031_IHC_M_19011506_601.jpg

GTX101408 IHC-P Image

Aldolase A antibody detects Aldolase A protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Aldolase A antibody (GTX101408) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf