GeneTex
United States (US)

Aldolase A antibody

GTX30044_20180530_ICCIF_18121410_658.jpg
GTX30044_20180530_WB_18121410_377.jpg

Cat No. GTX30044

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:100
Not tested in other applications.

Calculated MW

39 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-348 of human ALDOA (NP_908930.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ALDA Antibody , GSD12 Antibody , ALDOA Antibody

Background

The protein encoded by this gene, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES
GTX30044_20180530_ICCIF_18121410_658.jpg

GTX30044 ICC/IF Image

ICC/IF analysis of A549 cell using GTX30044 Aldolase A antibody.
Blue : DAPI

GTX30044_20180530_WB_18121410_377.jpg

GTX30044 WB Image

WB analysis of various samples using GTX30044 Aldolase A antibody.
Dilution : 1:1000
Loading : 25μg per lane