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Aldolase B antibody

Orthogonal
Anti-Aldolase B antibody used in Western Blot (WB). GTX101363
Anti-Aldolase B antibody used in Western Blot (WB). GTX101363
Anti-Aldolase B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101363
Anti-Aldolase B antibody used in Western Blot (WB). GTX101363
Anti-Aldolase B antibody used in IHC (Paraffin sections) (IHC-P). GTX101363
Anti-Aldolase B antibody used in Western Blot (WB). GTX101363
Anti-Aldolase B antibody used in Western Blot (WB). GTX101363

Cat. No. GTX101363

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Turtle
Package
100 μl ($399),
25 μl ($169)
See all Aldolase B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

Neuro2A , GL261 , C8D30 , PC-12 , mouse brain , rat brain , 293T , A431 , HeLa , HepG2

Predict Reactivity

Rabbit, Sheep, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.09 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Aldolase B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169399

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldolase, fructose-bisphosphate B , ALDB , ALDO2

Background

Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Aldolase B antibody used in Western Blot (WB). GTX101363

GTX101363 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase B antibody (GTX101363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Aldolase B antibody used in Western Blot (WB). GTX101363

GTX101363 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase B antibody (GTX101363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Aldolase B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101363

GTX101363 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Aldolase B(GTX101363) antibody at 1:500 dilution.

Anti-Aldolase B antibody used in Western Blot (WB). GTX101363

GTX101363 WB Image

Aldolase B antibody detects ALDOB protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
10% SDS-PAGE
Aldolase B antibody (GTX101363) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Aldolase B antibody used in IHC (Paraffin sections) (IHC-P). GTX101363

GTX101363 IHC-P Image

Aldolase B antibody detects ALDOB protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma tissue.
Aldolase B antibody (GTX101363) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Aldolase B antibody used in Western Blot (WB). GTX101363

GTX101363 WB Image

Aldolase B antibody detects ALDOB protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
10% SDS-PAGE
Aldolase B antibody (GTX101363) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Aldolase B antibody used in Western Blot (WB). GTX101363

GTX101363 WB Image

Orthogonal

The data was published in the journal PLoS One in 2013. PMID: 23874782

REFERENCE

Application Reference

Zhang Z et al. J Cell Mol Med 2024; 28 (3) : e18073 Dihydroxyacetone phosphate accumulation leads to podocyte pyroptosis in diabetic kidney disease.
Chen SM et al. PLoS One 2020; 15 (1) : e0227838 Effect of prednisolone on glyoxalase 1 in an inbred mouse model of aristolochic acid nephropathy using a proteomics method with fluorogenic derivatization-liquid chromatography-tandem mass spectrometry.
Application : WB
Reactivity : Human
Shimada M et al. Nutrition Research 2017; (Epub) Dietary supplementation with myo-inositol reduces hepatic triglyceride accumulation and expression of both fructolytic and lipogenic genes in rats fed a high-fructose diet.
Application : WB
Reactivity : Rat
Dawson NJ et al. PLoS One 2013; 8 (7) : e68830 Characterization of fructose-1,6-bisphosphate aldolase during anoxia in the tolerant turtle, Trachemys scripta elegans: an assessment of enzyme activity, expression and structure.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169