GeneTex
United States (US)

Aldolase B antibody

GTX101363_40751_WB_R_19011506_791.jpg
GTX101363_40751_20180105_WB_19011506_260.jpg
GTX101363_40751_20170713_WB_M_R_19011506_584.jpg
GTX101363_40751_WB_M_19011506_437.jpg
GTX101363_40751_IFA_19011506_833.jpg
GTX101363_40751_IHC_19011506_582.jpg
GTX101363_43068_20180105_WB_19011506_626.jpg

Cat No. GTX101363

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain , rat brain , Neuro2A , GL261 , C8D30 , PC-12

Predict Reactivity

Rabbit, Sheep, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.09mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Aldolase B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ALDB antibody, ALDO2 antibody, ALDOB antibody, fructose-bisphosphate aldolase B antibody, aldolase 2 antibody, aldolase B, fructose-bisphosphatase antibody, liver-type aldolase antibody, aldolase B, fructose-bisphosphate antibody

Background

Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101363_40751_WB_R_19011506_791.jpg

GTX101363 WB Image

Aldolase B antibody detects ALDOB protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
10% SDS-PAGE
Aldolase B antibody (GTX101363) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101363_40751_20180105_WB_19011506_260.jpg

GTX101363 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase B antibody (GTX101363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101363_40751_20170713_WB_M_R_19011506_584.jpg

GTX101363 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase B antibody (GTX101363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101363_40751_WB_M_19011506_437.jpg

GTX101363 WB Image

Aldolase B antibody detects ALDOB protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
10% SDS-PAGE
Aldolase B antibody (GTX101363) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101363_40751_IFA_19011506_833.jpg

GTX101363 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Aldolase B(GTX101363) antibody at 1:500 dilution.

GTX101363_40751_IHC_19011506_582.jpg

GTX101363 IHC-P Image

Aldolase B antibody detects ALDOB protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma tissue.
Aldolase B antibody (GTX101363) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101363_43068_20180105_WB_19011506_626.jpg

GTX101363 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase B antibody (GTX101363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169