APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , Jurkat , Raji , Mouse liver , PC-12 , Rat2 , mouse brain , rat brain
Predict Reactivity
Rabbit, Sheep, Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.3 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Aldolase B. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10623571
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
aldolase, fructose-bisphosphate B , ALDB , ALDO2
Background
Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX114914 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase B antibody (GTX114914) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
|
|
|
GTX114914 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase B antibody (GTX114914) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX114914 WB Image
Aldolase B antibody detects ALDOB protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 ug Rat2 whole cell lysate/extract
10 % SDS-PAGE
Aldolase B antibody (GTX114914) dilution: 1:1000
|
|
|
GTX114914 WB Image
Sample (20 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX114914 diluted at 1:10000
|
|
|
GTX114914 ICC/IF Image
Immunofluorescence analysis of methanol-fixed A431, using Aldolase B(GTX114914) antibody at 1:200 dilution.
|
|
|
GTX114914 IHC-P Image
Aldolase B antibody detects ALDOB protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma tissue.
Aldolase B antibody (GTX114914) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
|
GTX114914 WB Image
Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: JurKat
D: Raji
10% SDS PAGE
GTX114914 diluted at 1:1000
|
|
|
GTX114914 WB Image
Sample (30 μg of whole cell lysate)
A: zebrafish eye
10% SDS PAGE
GTX114914 diluted at 1:1000
|
|
|
GTX114914 IHC-P Image
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Aldolase B antibody (GTX114914) at 1:300 dilution.
|
REFERENCE
There are currently no references for Aldolase B antibody (GTX114914). Be the first to share your publications with this product.
REVIEW
Aldolase B antibody
Cat. No. GTX114914
|
Rating
|
|
( Average 4 based on 1 users reviews)
|
Application
|
Western Blot(WB)
|
|
( Average 4 based on 1 users reviews)
|
Date :
Anonymous submitted on 04-Aug-2016
Application Tested :
WB
Sample Species :
Rat
Sample :
liver tissue
Amount used :
40μg
Blocking :
5% skim milk, 20°C, 1Hr
Primary Antibody :
1:5000, 4°C, 16Hr
