GeneTex
United States (US)

Aldolase B antibody

GTX114914_40240_20170713_WB_M_R_18121410_783.jpg
GTX114914_40240_WB_R_18121410_288.jpg
GTX114914_40240_IHC_18121410_369.jpg
GTX114914_WB_18121410_323.jpg
GTX114914_40240_IFA_18121410_799.jpg
GTX114914_40240_WB_M_liver_18121410_874.jpg

Cat No. GTX114914

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , mouse liver , PC-12 , Rat2 , mouse brain , rat brain

Predict Reactivity

Zebrafish, Rabbit, Sheep, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Aldolase B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Aldolase, Fructose-Bisphosphate B,Aldb,Aldo2,Aldob

Background

Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX114914_40240_20170713_WB_M_R_18121410_783.jpg

GTX114914 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase B antibody (GTX114914) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114914_40240_WB_R_18121410_288.jpg

GTX114914 WB Image

Aldolase B antibody detects ALDOB protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 ug Rat2 whole cell lysate/extract
10 % SDS-PAGE
Aldolase B antibody (GTX114914) dilution: 1:1000

GTX114914_40240_IHC_18121410_369.jpg

GTX114914 IHC-P Image

Aldolase B antibody detects ALDOB protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma tissue.
Aldolase B antibody (GTX114914) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114914_WB_18121410_323.jpg

GTX114914 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: JurKat
D: Raji
10% SDS PAGE
GTX114914 diluted at 1:1000

GTX114914_40240_IFA_18121410_799.jpg

GTX114914 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using Aldolase B(GTX114914) antibody at 1:200 dilution.

GTX114914_40240_WB_M_liver_18121410_874.jpg

GTX114914 WB Image

Sample (20 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX114914 diluted at 1:10000

REVIEW

Aldolase B antibody Cat No. GTX114914

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 04-Aug-2016
Score :
Application Tested : WB
Sample Species : Rat
Sample : liver tissue
Amount used : 40μg
Blocking : 5% skim milk, 20°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169