Aldolase C antibody [N1C1]

Name: Aldolase C antibody [N1C1]
Brand: GeneTex
SKU: GTX102284
Price: 399.00 USD
Rating: 3 (1 reviews)

Anti-Aldolase C antibody [N1C1] used in IHC (Frozen sections) (IHC-Fr). GTX102284

Anti-Aldolase C antibody [N1C1] used in Western Blot (WB). GTX102284

Cat. No. GTX102284

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-Fr
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Aldolase C products

View product citations for antibody GTX102284 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-Fr	1:100-1:1000

Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

U87-MG , MCF-7 , mouse brain , rat brain

Predict Reactivity

Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Aldolase C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11178511

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldolase, fructose-bisphosphate C , ALDC

Background

This gene encodes a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively. [provided by RefSeq]

Database

Gene ID: 230 ALDOC
UniProt: P09972 ALDOC

Research Area

DATA IMAGES

GTX102284 IHC-Fr Image

Aldolase C antibody [N1C1] detects Aldolase C protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Aldolase C protein stained by Aldolase C antibody [N1C1] (GTX102284) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX102284 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase C antibody [N1C1] (GTX102284) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102284 WB Image

Sample (30 μg of whole cell lysate)
A: U87-MG
B: MCF-7
10% SDS PAGE
GTX102284 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102284 WB Image

The data was published in the journal BMC Genomics in 2015. PMID: 25652794

REFERENCE

Application Reference

Carla Proserpio et al. Cancers (Basel) 2022; 14 (9) MEOX2 Regulates the Growth and Survival of Glioblastoma Stem Cells by Modulating Genes of the Glycolytic Pathway and Response to Hypoxia.

Application : WB

Reactivity : Human

Lin LL et al. BMC Genomics 2015; 16 : 41 Integrating transcriptomics and proteomics to show that tanshinone IIA suppresses cell growth by blocking glucose metabolism in gastric cancer cells.

Application : WB

Reactivity : Human

Levine J et al. Glia 2015; Traumatically injured astrocytes release a proteomic signature modulated by STAT3-dependent cell survival.

Application : WB

Wu YH et al. PLoS One 2013; 8 (8) : e70642 Quantitative proteomic analysis of human lung tumor xenografts treated with the ectopic ATP synthase inhibitor citreoviridin.

Application : WB

Reactivity : Human

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