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Aldolase C antibody [N1C1]

Anti-Aldolase C antibody [N1C1] used in IHC (Frozen sections) (IHC-Fr). GTX102284
Anti-Aldolase C antibody [N1C1] used in Western Blot (WB). GTX102284
Anti-Aldolase C antibody [N1C1] used in Western Blot (WB). GTX102284

Cat No. GTX102284

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-Fr
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

NT2D1 , IMR32 , U87-MG , MCF-7 , mouse brain , rat brain

Predict Reactivity

Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Aldolase C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

aldolase, fructose-bisphosphate C , ALDC

Background

This gene encodes a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Aldolase C antibody [N1C1] used in IHC (Frozen sections) (IHC-Fr). GTX102284

GTX102284 IHC-Fr Image

Aldolase C antibody [N1C1] detects Aldolase C protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Aldolase C protein stained by Aldolase C antibody [N1C1] (GTX102284) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Aldolase C antibody [N1C1] used in Western Blot (WB). GTX102284

GTX102284 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase C antibody [N1C1] (GTX102284) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Aldolase C antibody [N1C1] used in Western Blot (WB). GTX102284

GTX102284 WB Image

Sample (30 µg of whole cell lysate)
A: U87-MG
B: MCF-7
10% SDS PAGE
GTX102284 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Aldolase C antibody [N1C1] Cat No. GTX102284

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 06-Feb-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : oral cancer cells
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169