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Alkaline Phosphatase (Tissue Non-Specific) antibody [2F4]

Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [2F4] used in IHC (Paraffin sections) (IHC-P). GTX60568
Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [2F4] used in Western Blot (WB). GTX60568
Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [2F4] used in Flow cytometry (FACS). GTX60568
Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [2F4] used in ELISA (ELISA). GTX60568

Cat. No. GTX60568

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2F4

Isotype

 IgG1

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all Alkaline Phosphatase (Tissue Non-Specific) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human Alkaline Phosphatase (Tissue Non-Specific) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_2887979

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alkaline phosphatase, biomineralization associated , AP-TNAP , APTNAP , HOPS , TNALP , TNAP , TNSALP

Cellular Localization

Cell membrane

Background

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]

Database

Research Area

DATA IMAGES

Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [2F4] used in IHC (Paraffin sections) (IHC-P). GTX60568

GTX60568 IHC-P Image

IHC-P analysis of ovarian cancer tissue using GTX60568 Alkaline Phosphatase (Tissue Non-Specific) antibody [2F4].

Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [2F4] used in Western Blot (WB). GTX60568

GTX60568 WB Image

WB analysis of HeLa (1), and NTERA-2 (2) cell lysate using GTX60568 Alkaline Phosphatase (Tissue Non-Specific) antibody [2F4].

Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [2F4] used in Flow cytometry (FACS). GTX60568

GTX60568 FACS Image

FACS analysis of MCF-7 cells using GTX60568 Alkaline Phosphatase (Tissue Non-Specific) antibody [2F4].
Green : Alkaline Phosphatase (Tissue Non-Specific)
Red : negative control

Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [2F4] used in ELISA (ELISA). GTX60568

GTX60568 ELISA Image

ELISA analysis of antigen using GTX60568 Alkaline Phosphatase (Tissue Non-Specific) antibody [2F4].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

REFERENCE

Application Reference

Chatterjee R et al. Eur J Cell Biol 2017; (Epub) Epigenetic and microenvironmental alterations in bone marrow associated with ROS in experimental aplastic anemia.
Application : FACS
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409