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Alkaline Phosphatase (Tissue Non-Specific) antibody [ALPL/597]

Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [ALPL/597] used in IHC (Paraffin sections) (IHC-P). GTX34402
Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [ALPL/597] used in IHC (Paraffin sections) (IHC-P). GTX34402
Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [ALPL/597] used in IHC (Paraffin sections) (IHC-P). GTX34402
Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [ALPL/597] used in IHC (Paraffin sections) (IHC-P). GTX34402

Cat No. GTX34402

Host Mouse
Clonality Monoclonal
Clone Name ALPL/597
Isotype IgG1
Application ICC/IF, IHC-P
Reactivity Human, Bovine
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 1:50-1:100 for 30 min at Room Temp

Note :

IHC-P
Boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Specificity/Sensitivity

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypo-phosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human ALPL protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Alkaline Phosphatase, Biomineralization Associated,Ap-Tnap,Aptnap,Hops,Tnalp,Tnap,Tnsalp,Alpl

Background

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]

Database

Research Area

DATA IMAGES
Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [ALPL/597] used in IHC (Paraffin sections) (IHC-P). GTX34402

GTX34402 IHC-P Image

Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).

Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [ALPL/597] used in IHC (Paraffin sections) (IHC-P). GTX34402

GTX34402 IHC-P Image

Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).

Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [ALPL/597] used in IHC (Paraffin sections) (IHC-P). GTX34402

GTX34402 IHC-P Image

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).

Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [ALPL/597] used in IHC (Paraffin sections) (IHC-P). GTX34402

GTX34402 IHC-P Image

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).

Package List Price ($)
$ 329