Alkaline Phosphatase (Tissue Non-Specific) antibody

Cat. No. GTX03390

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, ELISA
Reactivity	Human, Mouse, Rat
Package	100 μg ($439)

See all Alkaline Phosphatase (Tissue Non-Specific) products

Datasheet

View product citations for antibody GTX03390 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.25-0.5μg/ml
ICC/IF	5μg/ml
ELISA	0.1-0.5μg/ml

Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄

Preservative

0.01mg Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human Alkaline phosphatase/ALPL recombinant protein (Position: K229-H482).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alkaline phosphatase, biomineralization associated , AP-TNAP , APTNAP , HOPS , TNALP , TNAP , TNSALP

Cellular Localization

Cell membrane

Background

This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015]

Database

Gene ID: 249 ALPL
UniProt: P05186 ALPL

Research Area

DATA IMAGES

GTX03390 WB Image

WB analysis of various sample lysates using GTX03390 Alkaline Phosphatase (Tissue Non-Specific) antibody.
Lane 1 : HeLa whole cell lysates
Lane 2 : HEK293 whole cell lysates
Lane 3 : rat kidney tissue lysates
Lane 4 : RH-35 whole cell lysates
Lane 5 : mouse kidney tissue lysates
Lane 6 : HEPA1-6 whole cell lysates
Loading : 50μg
Dilution : 0.5 μg/mL

GTX03390 ICC/IF Image

ICC/IF analysis of A431 cells using GTX03390 Alkaline Phosphatase (Tissue Non-Specific) antibody.
Antigen retrieval : enzyme antigen retrieval reagent for 15 mins
Dilution : 5μg/mL

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 439

Qty