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Alkaline Phosphatase (Tissue Non-Specific) antibody

Orthogonal
Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody used in Western Blot (WB). GTX100817
Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody used in Western Blot (WB). GTX100817
Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody used in IHC (Paraffin sections) (IHC-P). GTX100817

Cat. No. GTX100817

Reference 13
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Sheep
Package
100 μl ($399),
25 μl ($169)
See all Alkaline Phosphatase (Tissue Non-Specific) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Cat, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Alkaline Phosphatase (Tissue Non-Specific). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949638

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alkaline phosphatase, biomineralization associated , AP-TNAP , APTNAP , HOPS , TNALP , TNAP , TNSALP

Cellular Localization

Cell membrane; Lipid-anchor , GPI-anchor

Background

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody used in Western Blot (WB). GTX100817

GTX100817 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody (GTX100817) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody used in Western Blot (WB). GTX100817

GTX100817 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody (GTX100817) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody used in IHC (Paraffin sections) (IHC-P). GTX100817

GTX100817 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using alkaline phosphatase (liver/bone/kidney)(GTX100817) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Soria-Tobar L et al. Alzheimers Res Ther 2024; 16 (1) : 233 Blockade of brain alkaline phosphatase efficiently reduces amyloid-β plaque burden and associated cognitive impairment.
Bernabei I et al. Int J Mol Sci 2023; 24 (11) CD11b Deficiency Favors Cartilage Calcification via Increased Matrix Vesicles, Apoptosis, and Lysyl Oxidase Activity.
Kengelbach-Weigand A et al. NPJ Regen Med 2021; 6 (1) : 49 Personalized medicine for reconstruction of critical-size bone defects - a translational approach with customizable vascularized bone tissue.
Yen Y.T. et al. Commun Biol. 2021; 4 (1) : 658 PP2A in LepR+ mesenchymal stem cells contributes to embryonic and postnatal endochondral ossification through Runx2 dephosphorylation.
Application : WB, IHC-P
Reactivity : Mouse
Ou L et al. Ecotoxicol Environ Saf 2021; 208 : 111668 Effects of cadmium on osteoblast cell line: Exportin 1 accumulation, p-JNK activation, DNA damage and cell apoptosis.
Application : WB
Reactivity : Mouse
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REVIEW

Alkaline Phosphatase (Tissue Non-Specific) antibody Cat. No. GTX100817

Rating
( Average 5 based on 2 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 2 users reviews)
Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody used in IHC (Paraffin sections) (IHC-P). GTX100817
Date : Anonymous submitted on 13-May-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Bone tissue
Fixation : 4% PFA in PBS, 4°C, 16Hr
Permeabilization /Antigen retrieval : 0.3% Triton X-100 in PBS, 25°C, 0.25Hr
Primary Antibody : 1:100, 4°C, 14Hr
Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody used in IHC (Paraffin sections) (IHC-P). GTX100817
Date : Anonymous submitted on 13-May-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Embryonic mouse bone
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : Tris-EDTA pH9, boil°C, 0.5Hr
Primary Antibody : 1:100, 4°C, 16Hr
Submit a Review
Application Reference
Soria-Tobar L et al. Alzheimers Res Ther 2024; 16 (1):233 Blockade of brain alkaline phosphatase efficiently reduces amyloid-β plaque burden and associated cognitive impairment.
Bernabei I et al. Int J Mol Sci 2023; 24 (11) CD11b Deficiency Favors Cartilage Calcification via Increased Matrix Vesicles, Apoptosis, and Lysyl Oxidase Activity.
Kengelbach-Weigand A et al. NPJ Regen Med 2021; 6 (1):49 Personalized medicine for reconstruction of critical-size bone defects - a translational approach with customizable vascularized bone tissue.
Yen Y.T. et al. Commun Biol. 2021; 4 (1):658 PP2A in LepR+ mesenchymal stem cells contributes to embryonic and postnatal endochondral ossification through Runx2 dephosphorylation.
Application : WB, IHC-P
Reactivity : Mouse
Ou L et al. Ecotoxicol Environ Saf 2021; 208:111668 Effects of cadmium on osteoblast cell line: Exportin 1 accumulation, p-JNK activation, DNA damage and cell apoptosis.
Application : WB
Reactivity : Mouse
Manal Matoug-Elwerfelli et al. Sci Rep 2020; 10 (1):21553 Ex-vivo recellularisation and stem cell differentiation of a decellularised rat dental pulp matrix.
Application : IHC-P
Reactivity : Human
Agustina H et al. Patholog Res Int 2018; 2018:6346409 The Role of Osteocalcin and Alkaline Phosphatase Immunohistochemistry in Osteosarcoma Diagnosis.
Chen YW et al. Materials Science and Engineering: C 2018; 91:679-687 Osteogenic and angiogenic potentials of the cell-laden hydrogel/mussel-inspired calcium silicate complex hierarchical porous scaffold fabricated by 3D bioprinting.
Application : WB
Reactivity : Human
Agustina H et al. Hindawi 2018; (Epub) The Role of Osteocalcin and Alkaline Phosphatase Immunohistochemistry in Osteosarcoma Diagnosis.
Application : IHC-P
Reactivity : Human
Shie MY et al. Materials Science and Engineering: C 2017; 73:726-735 Synergistic * in the osteogenic and angiogenic differentiation of human mesenchymal stem cells by calcium silicate–graphene composites.
Application : WB
Weigand A et al. Tissue Eng Part A 2016; (Epub) Bone tissue engineering under xenogeneic-free conditions in a large animal model as a basis for early clinical applicability.
Application : IHC-P
Reactivity : Human
Boos AM et al. Int J Nanomedicine 2014; 9:5317-39 Autologous serum improves bone formation in a primary stable silica-embedded nanohydroxyapatite bone substitute in combination with mesenchymal stem cells and rhBMP-2 in the sheep model.
Application : IHC-P
Reactivity : Sheep
Greenbaum AM et al. Blood 2012; 120 (2):295-302 N-cadherin in osteolineage cells is not required for maintenance of hematopoietic stem cells.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169