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Alkaline Phosphatase (Tissue Non-Specific) antibody

Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody used in IHC (Paraffin sections) (IHC-P). GTX100817
Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody used in Western Blot (WB). GTX100817

Cat. No. GTX100817

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC

Reactivity

Human, Mouse, Sheep
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Cat, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Alkaline Phosphatase (Tissue Non-Specific). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949638

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

alkaline phosphatase, biomineralization associated , AP-TNAP , APTNAP , HOPS , TNALP , TNAP , TNSALP

Cellular Localization

Cell membrane; Lipid-anchor , GPI-anchor

Background

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody used in IHC (Paraffin sections) (IHC-P). GTX100817

GTX100817 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using alkaline phosphatase (liver/bone/kidney)(GTX100817) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody used in Western Blot (WB). GTX100817

GTX100817 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody (GTX100817) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Alkaline Phosphatase (Tissue Non-Specific) antibody Cat. No. GTX100817

Rating ( Average 5 based on 2 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 13-May-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Bone tissue
Fixation : 4% PFA in PBS, 4°C, 16Hr
Permeabilization /Antigen retrieval : 0.3% Triton X-100 in PBS, 25°C, 0.25Hr
Primary Antibody : 1:100, 4°C, 14Hr
Date : Anonymous submitted on 13-May-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Embryonic mouse bone
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : Tris-EDTA pH9, boil°C, 0.5Hr
Primary Antibody : 1:100, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169