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Alkaline phosphatase antibody [HL1267]

Recombinant antibody Orthogonal
Anti-Alkaline phosphatase antibody [HL1267] used in Western Blot (WB). GTX636669
Anti-Alkaline phosphatase antibody [HL1267] used in Western Blot (WB). GTX636669
Anti-Alkaline phosphatase antibody [HL1267] used in IHC (Paraffin sections) (IHC-P). GTX636669

Cat No. GTX636669

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1267

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse
Package
100 μl ($369),
25 μl ($169)
See all Alkaline phosphatase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Alkaline phosphatase. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910037

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

alkaline phosphatase, intestinal , IAP

Background

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-Alkaline phosphatase antibody [HL1267] used in Western Blot (WB). GTX636669

GTX636669 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase antibody [HL1267] (GTX636669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Alkaline phosphatase antibody [HL1267] used in Western Blot (WB). GTX636669

GTX636669 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase antibody [HL1267] (GTX636669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Alkaline phosphatase antibody [HL1267] used in IHC (Paraffin sections) (IHC-P). GTX636669

GTX636669 IHC-P Image

Alkaline phosphatase antibody [HL1267] detects Alkaline phosphatase protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Alkaline phosphatase stained by Alkaline phosphatase antibody [HL1267] (GTX636669) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Steiner D et al. Cells 2022; 11 (6) Impact of Endothelial Progenitor Cells in the Vascularization of Osteogenic Scaffolds.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

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Package List Price ($)
$ 369
$ 169
Custom Conjugations