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Alkaline phosphatase (intestinal) antibody [3B10E7]

Anti-Alkaline phosphatase (intestinal) antibody [3B10E7] used in ELISA (ELISA). GTX60746
Anti-Alkaline phosphatase (intestinal) antibody [3B10E7] used in Western Blot (WB). GTX60746
Anti-Alkaline phosphatase (intestinal) antibody [3B10E7] used in Western Blot (WB). GTX60746
Anti-Alkaline phosphatase (intestinal) antibody [3B10E7] used in Flow cytometry (FACS). GTX60746

Cat. No. GTX60746

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3B10E7

Isotype

 IgG1

Application

 WB, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all Alkaline phosphatase (intestinal) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human Alkaline phosphatase (intestinal) (AA: 397-458) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_2887999

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alkaline phosphatase, intestinal , IAP

Background

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-Alkaline phosphatase (intestinal) antibody [3B10E7] used in ELISA (ELISA). GTX60746

GTX60746 ELISA Image

ELISA analysis of antigen using GTX60746 Alkaline phosphatase (intestinal) antibody [3B10E7].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-Alkaline phosphatase (intestinal) antibody [3B10E7] used in Western Blot (WB). GTX60746

GTX60746 WB Image

WB analysis of HL60 (1) and HepG2 (2) cell lysate using GTX60746 Alkaline phosphatase (intestinal) antibody [3B10E7].

Anti-Alkaline phosphatase (intestinal) antibody [3B10E7] used in Western Blot (WB). GTX60746

GTX60746 WB Image

WB analysis of HEK293 (1) and ALPI (AA: 397-458)-hIgGFc transfected HEK293 (2) cell lysate using GTX60746 Alkaline phosphatase (intestinal) antibody [3B10E7].

Anti-Alkaline phosphatase (intestinal) antibody [3B10E7] used in Flow cytometry (FACS). GTX60746

GTX60746 FACS Image

FACS analysis of HepG2 cells using GTX60746 Alkaline phosphatase (intestinal) antibody [3B10E7].
Green : Alkaline phosphatase (intestinal)
Purple : negative control

REFERENCE

Application Reference

Bing T et al. Adv Sci (Weinh) 2019; 6 (11) : 1900143 Aptameric Probe Specifically Binding Protein Heterodimer Rather Than Monomers.
Tao Bing et al. Advanced Science 2019; (Epub) Aptameric Probe Specifically Binding Protein Heterodimer Rather Than Monomers.
Application : FACS
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409